4 86 
Journal of Agricultural Research 
Vol. XI, No. io 
parasitology, since all were found to be identical organisms. For the 
sake of convenience in presentation each of the strains was given a sepa¬ 
rate number as follows: Strain 286 was isolated on October 26, 1914, 
from a Lima bean pod collected from a small patch at Vineland, N. J. 
Stylospores and pycnospores were abundant. Strain 420 was isolated 
from a Lima bean pod collected during the fall of 1914 by Mr. M. B. 
Waite in Maryland. Stylospores, as well as pycnospores, were present 
in abundance. Strain 447 was isolated from material collected on 
January 1, 1915, from the same field as No. 286. Strain 567 is a reisola¬ 
tion made on April 12, 1915, from a pod that had been inoculated in the 
greenhouse on March 13, 1915, with strain 447. Pycnospores were 
abundant,*but there were no stylospores. Strain 598, as previously 
stated, resulted from the isolation on June 4, 1915, of a single ascospore 
from a pod that had been wintered out at Washington, D. C. Strain 
601 was isolated on August 7, 1915, from leaves of Lima beans collected 
at Cape May, N. J., on August 5, 1915. 
Other isolations of the same organism not used for inoculation pur¬ 
poses have also been made for the purpose of identification and com¬ 
parison. Frequent reisolations were made from inoculated pods, one 
strain (567) of which was used to make inoculations. From specimens 
inoculated with this strain the causal fungus was again recovered, thus 
fulfilling the requirement of Koch's postulates, claimed necessary to 
prove parasitism. 
The first infection experiments were made on January 15, 1915, when 
20 nearly mature pods on six small plants were inoculated, 10 with 
organism 286 and 10 with organism 420. After the pods were inoculated 
by inserting spores and hyphae, the plants were sprayed with spores 
suspended in water. They were then kept in a paper-covered infection 
cage for 24 hours. On February 20, pycnidia were abundant on the 
stems and on six pods inoculated with No. 286. Of those inoculated 
with No. 420, five pods were dead, and pycnidia were already on four 
and developed on one more after 10 days in a moist chamber. In this 
case there were no leaf infections, though the stems, particularly the 
lower parts, were covered with pycnidia, the pycnidia developing most 
abundantly on the moribund stems and pods. 
On March 13, 21 small plants were sprayed with organism 447. There 
were only three small, immature pods in all and they were inoculated by 
inserting spores and hyphae. These plants were inclosed for 48 hours 
in paper-covered infection cages. Twelve days later the three pods were 
drying up, and pycnidia were present on one and developed on the two 
others after 7 days in a moist chamber. Here again no leaves were 
infected, and the pods did not show typical infections. These results 
were disappointing at the time, but it was discovered later that young 
leaves and pods were difficult to infect, mature parts being much more 
susceptible to the disease. 
