488 
Journal of Agricultural Research 
Vol. XI, No. io 
and all but one bore numerous pycnidia by August 9. Cultures from some 
of these infected pods produced a growth characteristic of the pycnidial 
stage. On the same date spores of organism 598 were smeared on the 
surface of five nearly full-grown pods. There were no signs of infection 
on June 21, but by July 7 three showed evidence of infection, two remained 
sound. Seven pods of various ages were sprayed with organism 598 and 
confined in an infection cage for 30 hours. Pycnidia appeared on one of 
these pods on June 21 and on two others on August 9 as well as on the 
stems and some of the dead branches. The writer was away for sometime 
previous to August 2, and it is not definitely known just how the disease 
developed. It is probable, in view of the fact that the pods were dead 
and studded all over with pycnidia, that they succumbed sometime 
earlier. 
On June 21 twenty-one pods were inoculated with organism 598 in the 
usual way. One diseased pod was removed on June 29, eighteen on July 
7, and one on August 17. One of the inoculated pods could not be found, 
and was therefore regarded as not infected. There were ten checks held 
for this experiment, one of which became infected and the only one of all 
the checks (66) held at various times. 
On August 17, ten pods were inoculated with organism 601, and by 
September 3 six were well covered with pycnidia. Four remained sound. 
Of 19 pods inoculated with 601 on September 7, ten showed typical in¬ 
fection on September 25. Nine remained sound. 
A summary of the results is given in Table I. 
Table) I .—Summary of inoculation experiments of Lima beans 
Organism No. 
Date of in¬ 
oculation. 
How inoculated. 
Number in¬ 
oculated. 
Num¬ 
ber in¬ 
fected. 
Num¬ 
ber of 
checks. 
Num¬ 
ber of 
checks 
in¬ 
fected. 
286, 
1915 * 
Jan. 15 
420, 
447 
. .do_ 
Mar. 13 
447 . 
447 . 
447 . 
Fragments of peri- 
thecia contain¬ 
ing asci from 
pods. 
598 
Apr. 
Apr. 
Apr. 
June 
June 
June 
. .do. 
9 
20 
23 
2 
3 
14 
598 
598 
601 
601 
. .do- 
June 21 
Aug. 17 
Sept. 7 
By insertion of 
spores in a wound. 
_do. 
Sprayed. 
Wounding. 
By wounding. 
Spraying. 
By wounding. 
_do. 
_do. 
_do. 
Spores smeared on 
pods. 
Sprayed. 
By wounding. 
_do. 
_do. 
10 pods.... 
. .do. 
21 plants... 
3 pods. 
12 pods.... 
9 pods. 
5 pods. 
21 pods.... 
15 pods.... 
17 pods.... 
5 pods. 
7 pods. 
21 pods.... 
1opods 
19 pods.... 
6 
5 
o 
5 
o 
o 
4 
17 
5 
6 
5 
5 
o 
o 
o 
o 
o 
o 
14 
16 
3 
6 
6 
o 
o 
3 
20 
6 
10 
1 
10 5 
o 
