706 
Journal of Agricultural Research 
Vol. XI, No. 13 
about five minutes, when she lay down. She gradually grew weaker, 
exhibiting a repetition of the same symptoms. On the morning of 
October io she could still stand for a few minutes. She was killed at 
9 a. m. on that day, and an autopsy was made. The autopsy showed 
nothing that could be considered abnormal. The records of temperature, 
pulse, and respiration showed nothing that could be considered specifically 
abnormal. 
LETHAL BOSE 
The experiments were too few in number to make it possible to speak 
positively in regard to the lethal dose. The results, however, are sig¬ 
nificant. 
Sheep. —Excluding No. 310, which was fed on the dry plant, consider¬ 
ing No. 369 as a case of death, for this animal would have died, without 
doubt, and adding the amounts of the two feedings of No. 309 for reasons 
which will appear later, the average lethal dose was 6.05 pounds per 
hundredweight of animal, with a minimum of 5 pounds and a maximum 
of 7.1 pounds. The variation from the average is so little that it is 
probably safe to say that the lethal dose for sheep is about 6 per cent of 
the animal’s weight. 
Cattle. —Tor the cattle the average lethal dose of the two fatal cases, 
Nos. 668 and 699, is 94.2 pounds per 1,000 pounds of animal. If it can be 
assumed that these are average cases, it would appear that the lethal dose 
for cattle is about 10 per cent of the animal’s weight. Cattle apparently 
are somewhat less susceptible to the poison than sheep. 
TOXIC BOSE 
The experiments with the sheep failed to show any difference between 
the lethal and toxic doses. 
In the case of the cattle, No. 699 showed symptoms on 82.6 pounds per 
1,000 pounds of animal, and succumbed after 106 pounds, and No. 663 
showed symptoms after 123.8 pounds of autoclaved material. No. 700 
exhibited symptoms after 56 pounds, but in this case there was a com¬ 
plication because of the animal’s weakness from lack of food. It appears 
then that the margin between the toxic and lethal limits is quite narrow. 
EUPATORIUM POISONING CUMULATIVE 
While there was some evidence that the toxic dose was smaller when 
the material was given in a short time, this difference was comparatively 
slight. It appears from the experimental cases that the elimination of 
the toxic substance takes place very slowly, so that there is a distinct 
cumulative effect. This was evident both in the cattle and in the sheep. 
This may explain the fact that sheep 309 was killed in a forced feeding 
of only 1.6 pounds per hundredweight on November 2, 1914. From 
October 24 to 26 it had received 4.4 pounds with no symptoms. The 
