Sept. 2, 1918 
Comparative Toxicity of Cottonseed Products 
439 
Table IV .—Percentage composition of the poultry diets 
Feed. 
Lot 1 
($ hens). 
Lot 2 
(5 hens). 
Lot 3 
(s hens). 
Lot 4 
(s hens). 
Lot s 
(5 hens). 
Lot 6 
(6 hens). 
Gossypol. 
O. 2 
Raw cottonseed kernels. 
30 
Ether-extracted cottonseed 
kernels. 
40 
Cottonseed meal, cooked two 
hours. 
40 
3 °~° 
O-30 
10 
30 
10 
10 
10 
26. 3 
10. 0 
30 
Cottonseed meal, cooked 28 
minutes. 
Meat meal. 
20-0 
20-30 
30 
19. 8 
10. 20 
IO 
20 
IO 
20 
IO 
Corn meal. 
Wheat bran. 
40-50 
30 
20 
20 
40-5° 
Ground oats. 
Whole milk powder. 
Skim-milk powder. 
20-10 
20-10 
Protein (NX 6.25). 
Protein from cottonseed. 
Cracked limestone. 
Tap water. 
23-3 
* 3 * 3 
l8. O 
IO. O 
29. 0 
18.8 
28.0 | 26.0 
0.0 1 9.3 
j-Ad libitum. 
The meat meal (80-85 per cent crude protein) was discontinued in lot 
5 after 32 days, as the birds had declined greatly. Additional milk 
powder (10 per cent) and additional corn meal (10 per cent) were sub¬ 
stituted in an attempt to make the diet more appetizing. This reduced 
the protein content to about 17 per cent. 
Lot 6 was started after all in lot 5 had died. 
Owing to inability to secure enough milk powder, after 140 days the 
amounts used in lots 1 and 4 were reduced to 10 per cent. Later, it was 
necessary to make use of skim-milk powder plus butter. 
After the pig, rat, and rabbit experiments had shown considerable 
difference in toxicity between the long-cooked and short-cooked meals, 
the hens in lot 2 were fed on the short-cooked meal from the eighty-fifth 
day in place of the long-cooked meal. 
The protein content of 40 per cent of extracted kernels is equal to 
about that of 55 per cent of cottonseed meal used in lots 1, 2, and 3. 
The fowls were fed under ordinary conditions by an experienced 
poultryman. Each lot was confined in outdoor turf-covered pens about 
6 by 30 feet. Covered sheds opening to the south were provided for roost¬ 
ing and shelter. The fowls were gradually worked up to the maximum 
amount of feed given each lot (0.9 pound) daily. Weighings were made 
every tenth day. The dead fowls were examined by Dr. B. F. Kaupp, 
Poultry Expert and Pathologist of this Station* under whose supervision 
the experiment was conducted. The results of this experiment are 
given in Table V. 
70392°—18 - 4 
