210 
Journal of Agricultural Research 
Vol. XIV, No. 5 
The spore may or may not be constricted at the septum. The spores of 
various forms, differing greatly in type, are straight or curved, and often 
larger at the ends than at the middle (fig. 3). 
INOCULATION EXPERIMENTS 
Podspot was reproduced with the characteristic symptoms on several 
varieties of okra and the organism recovered. It was again reproduced 
by inoculation from cultures resulting from such reisolations. 
f The original cultures were obtained in the fall of 1916, but the first 
inoculations were not made till some months later. Since okra does not 
grow well during the winter months under greenhouse conditions, plants 
were not started there till the spring of 1917. About August 1 abundant 
pods on these plants were suitable for inoculation. On August 10 a 
number were inoculated by inserting spores and hyphse into wounds 
made with a dissecting needle. A few infections resulted from the 
inoculations, and the 
characteristic symp¬ 
toms of the disease 
were produced. That 
a larger percentage of 
infection did not result 
was found later to be 
due to the fact that the 
pods were in most’cases 
too mature when in¬ 
oculated, young pods 
being much more sus¬ 
ceptible. On August 
Fig. 3. — Ascochyta abelmoschi: A number of pycnospores, some of 15, pods in different 
x“t, 0 are 5ePtated ' Sh0Wing ^ Variati ° nS “ ShaPe “ d Si “‘ stages of development, 
of five different varie¬ 
ties, New Lady Finger, Perkins Long Pod, Kleckleys Favorite, Dwarf Pro¬ 
lific, and White Velvet, were inoculated outdoors at the Arlington Experi¬ 
mental Farm as above with the same organism. Over 70 per cent of 
these pods developed the characteristic symptoms of the disease, the 
older pods remaining healthy or showing only slight infection. On 
August 20, pods, one-third to full grown but not mature, were inocu¬ 
lated in the greenhouse and 65 per cent became infected. 
The organism was recovered from the infected pods of the first inocula¬ 
tion and when fruiting abundantly was used in the greenhouse to inocu¬ 
late pods in all stages of development. In some cases several pods on 
the same plant were inoculated. The results showed that the upper or 
younger pods were the first to show symptoms of the disease, and the 
spots enlarged more rapidly. The infected spot on the pod next below 
developed more slowly, while the pods lowest down on the stem either 
remained healthy or showed but slight evidence of disease and then 
only at the wound. 
