536 
Journal of Agricultural Research 
Vol. XIV, No. la 
older cultures the medium becomes dark brown, and after 2 or 3 months the growth 
is discolored. 
Coagulated cow serum, —There is a surface membranous growth with little 
tendency to form aerial hyphae. Slow liquefaction of the medium occurs after 2 or 3 
weeks. 
Bouillon. —The medium is not rendered cloudy, but a fluffy growth appearing 
like small pieces of cotton develops in the bottom of the tube. In many tubes a rather 
firm membrane covers the surface of the medium. Aerial hyphae do not appear except 
in old cultures. 
Milk. —There is a slow digestion of this medium, three or four weeks being required 
before there is complete clearing. The reaction remains unchanged. A whitish 
surface membrane is formed. 
Indol is not produced. Dextrose, lactose, and saccharose are not fermented with 
the production of either alcohol or gas. 
THE DISEASE IN CATTLE 
Little is known of the disease in cattle resulting from natural infection. 
The source of infection and the manner of transmission are quite likely 
the same as in the human cases. However, to judge from the results 
obtained from experimental inoculations in cattle, these animals are not 
nearly so susceptible subjects as man. 
So far as is known at present, the lesions observed in cattle at the 
time of slaughter in the abattoir appear to be confined largely to the 
bronchial and mediastinal lymph glands. These tissues may be the 
seat of large areas of suppuration or several smaller purulent foci, all of 
which are usually surrounded by considerable granulation tissue and a 
fibrous capsule. Upon incising an affected gland there may be squeezed 
out a thick yellowish and tenacious pus which at once suggests actino¬ 
mycosis. In fact, the similarity of the lesions produced in the lymph 
glands by Coccidioides immitis and Actinomyces is so striking that the 
one affection may be easily mistaken for the other upon gross inspection 
alone. However, microscopic examination of fresh smears of pus at 
once establishes a diagnosis; in the one case spheres in various stages of 
development are present in quite large numbers, and in the other the 
colonies of the ray fungus are detected. 
INOCULATIONS OF EXPERIMENTAL ANIMALS 
Successful inoculations were made with guinea pigs, rabbits, dogs, 
cattle, sheep, and swine, the degree of susceptibility in these animals 
varying in about the order named. Rapid generalization of the disease 
usually followed intravenous inoculations, and in the guinea pig and dog 
subcutaneous inoculation proved fatal in a rather brief period. The 
lesions most frequently encountered are in the form of miliary or sub- 
miliary nodules or abscesses involving practically all the internal organs. 
The histological structure of the nodules is almost identical with that 
produced by tubercle bacilli—that is, epithelioid cells and a peripheral zone 
of lymphocytes with giant cells and central caseation. Inclosed in 
