Sept. x6» 1918 Occurrence of Coccidioidal Granuloma in Cattle 
537 
most of the giant cells one or more of the parasites can usually be seen. 
Many parasites are also observed lying free in the tissue. 
A considerable number of guinea pigs and rabbits were used in the 
inoculation tests, but a report on a few typical cases will suffice to show 
the general character of the lesions produced by the fungus recovered 
from cattle. 
Guinea pig i, —On January 19, 1916, guinea pig 1 was injected subcutaneously with 
1 cc. of a suspension in normal salt .solution of purulent material taken from bovine 
glands. After about a week a swelling developed at the point of injection, and later 
there was ulceration of the skin over this area with the formation of a scab. The 
animal gradually failed and died on April 1 in an emaciated condition. The autopsy 
revealed the presence of a local ulcer from which a scanty discharge has escaped, mat¬ 
ting the surrounding hairs. This lesion is partially scabbed over, and beneath the 
scab there is considerable thick yellowish pus in which many spheres are found on 
microscopic examination. On the floor of the sternum there are two rather large 
grayish nodules. There are several small nodules in the lungs and spleen. Parasites 
were demonstrated microscopically in teased preparations from these lesions. Cultures 
of the mold were obtained from the suprasternal nodule. 
Guinns pig 2. —On April 3, 1916, guinea pig 2 was injected subcutaneously with 
purulent material from guinea pig 1. It died on June 13. At the autopsy there 
was observed a local lesion as in the first case; both precrural lymph glands were 
enlarged and on being sectioned they showed abscess cavities of considerable size 
containing typical, thick, yellowish pus. On the left side the abscess had broken 
through the skin. The inguinal, sublumbar, suprasternal, subcostal, and bronchial 
lymph glands were also involved, all showing suppurating centers of greater or less 
proportions. Miliary nodules were distributed throughout both spleen and lungs 
(PI. 59, C). Parasites were demonstrated microscopically in lesions, and cultures 
were obtained. 
Guinea pig 3/ —On July 26, 1916, guinea pig 3 was injected intraperitoneally with 
1 cc. of a cloudy suspension in a normal salt solution of a 2-month-old culture con¬ 
taining many spores. The animal died on September 3. At the autopsy there was 
observed a rather large abscess in the folds of the great omentum, marked purulent 
periorchitis, and uniformly distributed miliary nodules in spleen and lungs. Parasites 
were demonstrated in both fresh and histological preparations. Cultures were 
obtained. 
Rabbit i. —On July 26, 1916, 1 cc. of material used in the preceding case was 
injected into the ear vein of rabbit 1. It died on September 17. At the autopsy 
there are observed miliary foci in lungs, liver, spleen, and kidneys. Similar lesions 
are found subpleurally and subperitoneally. Parasites were demonstrated in both 
fresh and histological preparations (PI. 60, A). Cultures were obtained. 
* Cauves 177 and 184. —On April 3, 1916, calves 177 and 184 were injected sub¬ 
cutaneously on the left side of the neck with 4 cc. of normal salt solution suspension 
of splenic nodules and purulent material from local lesion of guinea pig 1. In the 
course of a week both animals developed a local swelling about 75 or 100 mm. in diam¬ 
eter. After several weeks a small ulcer was formed from which a slight amount of 
discharge oozed, gluing together the hairs below the lesion. The ulcer soon scabbed 
over, and very shortly the skin showed complete healing. 
On September 26 calf 184 was killed, and an autopsy was performed. The carcass 
was in fair condition, and no lesions except the one at the point of injection were 
found. On being sectioned the local lesion was found to consist of a rather dense layer 
of fibrous tissue disposed peripherally inclosing a zone of granulation tissue with a 
purulent center. Many spherical bodies were demonstrated microscopically in fresh 
