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Journal of Agricultural Research 
Vol. XIV, No. is 
preparations of purulent material from the lesion, and in histological preparations these 
forms were observed both inclosed in giant cells and lying free in the granulation 
tissue (PI. 60, B, C). Cultures were obtained from this case. 
On November 9, 1917, calf 177 was killed and a post-mortem examination made. 
The carcass was in a very well nourished condition. The lesion at the point of in¬ 
jection had almost disappeared, there remaining only a small indurated tumor under 
the skin which on being sectioned showed a few yellowish foci containing thick pu¬ 
rulent matter surrounded by dense fibrous tissue. Parasites were present in the pus. 
No other lesions were found. 
Dog 258.—On October 19, 1916, dog 258 was injected intravenously with 1 cc. 
of a cloudy suspension of an old agar culture in normal salt solution. In about a week 
the animal showed symptoms of dyspnea, which rapidly became very much worse, 
the dog being found dead on October 29. The autopsy revealed the presence 
of miliary nodules uniformly distributed throughout both lungs; no other lesions were 
found. Large numbers of spherical bodies were demonstrated in freshly teased 
preparations of the nodules. Cultures were obtained. 
Dog 249.—On October 19, 1916, dog 249 (much larger than dog 258) was injected 
intravenously with 2 cc. of the above suspension. This animal developed symptoms 
similar to dog 258, but slighter later, it appearing to show somewhat greater resist¬ 
ance. Death occurred on November 2. At autopsy lesions similar to those in dog 
258 were found. Parasites were demonstrated microscopically. Cultures were ob¬ 
tained. 
Dog 326.—On October 19, 1916, dog 326 was injected with 2 cc. of above suspen¬ 
sion subcutaneously behind its right shoulder. In the course of a week or two a 
rather extensive swelling developed at the point of inoculation. The hair came off 
in a considerable area over the swelling, and an ulcer formed in the skin at this point, 
which after a time scabbed over. The extension of the disease from the primary 
lesion progressed gradually, the condition of the animal became steadily worse, and 
death occurred on December 18. At the autopsy extensive ulceration of the skin 
and deeper tissues was observed at the point of injection. The dependent subcu¬ 
taneous and intermuscular tissues showed considerable infiltration with inflamma¬ 
tory exudate. Both prescapular glands were enlarged. The lungs, liver, and kid¬ 
neys were the seat of miliary nodules. There is a nodule about 12 mm. in diameter 
present in the suprasternal region. A few parasites were demonstrated microscop¬ 
ically in stained sections of the lung nodules. 
Sheep 559.—On October 19, 1916, sheep 559 was injected with 3 cc. of above 
suspension intravenously. The animal died on June 17, 1917. At the autopsy the 
carcass was found in a fairly well nourished condition. The superficial tissues in 
the region of the left shoulder and the right side of the body from the shoulder to 
the flank showed a rather marked serosanguineous infiltration resulting from injuries 
inflicted by cattle kept in the same pen. Both submaxillary, both prescapular, 
both superficial inguinal, the bronchial, and mediastinal lymph glands had small 
abscess cavities containing yellowish sticky pus. The lungs were the seat of a severe 
bronchopneumonia with uniformly distributed miliary nodules and larger caseous 
encapsulated lesions. There was considerable pleuritis, the visceral pleura being 
greatly thickened with fibro-plastic exudate and adherent in many places to the 
parietal pleura. There were many miliary nodules in the liver and a few nodules 
in the kidneys. The parasite was demonstrated microscopically in fresh prepara¬ 
tions from lesions in lymph glands, lung, and liver. 
Sheep 560.—On October 19, 1916, sheep 560 was injected with 5 cc. of the above 
suspension subcutaneously behind its right shoulder. The animal died on July 14, 
1917. At the autopsy the carcass was found to be severely bruised; the local lesion, 
if any was present, being completely obscured by the extensive bruised condition. 
