Sept. x6.1918 Occurrence of Coccidioidal Granuloma in Cattle 
539 
The superficial lymph glands appeared free from infection. The lungs showed 
numerous small caseo-calcareous nodules, well encapsulated, produced by infestation 
with Strongylus ovis pulmonaris. The bronchial and mediastinal lymph glands were 
normal. The liver, intestines, and mesenteric glands showed severe infestation with 
Esophagostoma columbianum. Microscopic examination of the various tissues failed 
to reveal the presence of species of Coccidioides. 
Cai,f 176. —On October 19, 1916, calf 176 was injected with 4 cc. of above suspen¬ 
sion intravenously. In about a week the animal had marked symptoms of dyspnea, 
and appetite began to fail. The condition became rapidly worse, and the calf died 
on November 1. The autopsy revealed the presence of miliary nodules uniformly 
distributed in both lungs. The bronchial and mediastinal glands were enlarged. 
No other lesions were present. The parasite was demonstrated microscopically in 
teased preparations from the lung nodules. 
CaIvF 181.—On October 19, 1916, calf 181 was injected with 5 cc. of the above sus¬ 
pension subcutaneously on the right side of its neck. The local lesion produced in 
this case was very similar to that noted for calf 177. The animal was killed on Novem¬ 
ber 9, 1917, and a post-mortem examination made. No abnormalities were found, 
with the exception of the local lesion, which was essentially the same in character 
as that reported in calf 177. Parasites were demonstrated in pus from this lesion. 
Pig 3059.—On October 19, 1916, the right marginal ear vein of pig 3059 was injected 
with 2 cc. of the above suspension. After about two weeks small warty growths 
appeared on the surface of the injected ear, showing first in the immediate vicinity 
of the marginal vein. Somewhat later this warty appearance was observed over a 
considerable area of the ear which was markedly enlarged and drooping. The general 
condition of the animal remained very good. This animal was killed on July 17, 1917, 
and an autopsy made. The injected ear showed a number of small subcutaneous 
abscesses located chiefly in the region over the marginal vein. The superficial lymph 
glands were not involved; nor were the bones. The lungs were the seat of miliary 
nodules. Numerous small nodules were present in the liver, and there were a few in 
the spleen. The bronchial, mediastinal, and portal glands showed slight lesions. 
The parasites were demonstrated microscopically in the pus from the ear lesions and 
in the lung nodules. 
Pig 3053.—On October 19, 1916, the right marginal ear vein of pig (sow) 3053 was 
injected with 2 cc. of the above suspension. Lesions similar to those described for 
pig 3059 were also noted in this animal. It was observed that for the first few months 
following the inoculation the ear lesions gradually became worse. Then there was a 
considerable period in which little change was apparent, and finally there began a 
recession of the growths. When the animal was killed, on November 9, 1917, no trace 
of the ear lesions was found at the autopsy. Complete spontaneous healing had taken 
place. The lungs, however, were the seat of uniformly distributed nodules. These 
when examined histologically closely resembled tubercle nodules in structure. Para¬ 
sites were demonstrated in small numbers in the lesions, some appearing to be under¬ 
going degeneration. 
On August 10, 1917, this sow farrowed a litter of four pigs, three of which lived, and 
were kept with the mother until November 9. On February 20, 1918, these three pigs 
were slaughtered. The autopsies revealed no lesions in any of the three cases. 
ALLERGIC AND SEROLOGICAL TESTS NEGATIVE 
With a view to determining whether animals affected with the disease 
would respond to allergic tests, material for injection was prepared in 
the following manner: A cloudy suspension in normal salt solution of 
hyphae and spores from an old agar culture was autoclaved for 15 minutes 
