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Journal of Agricultural Research 
Vol. XIV, No. ia 
at 15 pounds' pressure and subsequently placed in a shaking apparatus 
and shaken for three hours. On July 17, 1917, calves 177 and 181 were 
injected subcutaneously with 5 cc. of this material. The temperatures 
of the animals prior to injection were normal, and during the next 48 
hours neither rise in temperature nor local reaction was noted. 
On September 11 the test was repeated, using as injection material 5-cc. 
doses of a sterilized, filtered, and concentrated (one-tenth original volume) 
bouillon culture grown for about 6 weeks at 37 0 C. Negative results 
were again obtained. 
Serums from calves 177 and 181 and from pig 3059 were subjected to 
both complement fixation and agglutination tests. In the complement- 
fixation tests two antigens were employed, antigen 1 being the same as 
the material used in the first allergic test, antigen 2 consisting of some 
of the substance employed in the second allergic test. No complement¬ 
fixing bodies were demonstrated in either case. Tor agglutination fluid 
antigen 1 was used. No specific agglutinins were detected. 
The negative results obtained in our allergic, complement-fixation, and 
agglutination tests correspond to those reported by Cooke (r), who states 
that in a human case no specific complement-fixing bodies or agglutinins 
could be found in the blood serum, using cultures of Coccidioides immiiis 
and emulsions of the same organism from human lesions as antigens. He 
also states that no specific skin reaction could be demonstrated. 
CONCLUSIONS 
(1) Coccidioidal granuloma (oidiomycosis) has been observed in cattle 
as a natural infection of the bronchial and mediastinal lymph glands. 
(2) The disease is transmissible experimentally to guinea pigs, rabbits, 
dogs, cattle, sheep, and swine. 
(3) Cattle affected with this disease show no response to subcautaneous 
allergic tests. 
(4) Neither specific complement-fixing bodies nor agglutinins are 
detectable in the serums of affected animals. 
LITERATURE CITED 
(1) Cooke, Jean V. 
1915. immunity tests in coccidioidal granuloma. In Arch. Int. Med., v. 
15, no. 3, p. 479-486. 
(2) Dickson, E. C. 
1915. OIDIOMYCOSIS IN CALIFORNIA WITH SPECIAL REFERENCE TO COCCIDIOIDAL 
GRANULOMA, INCLUDING NINE NEW CASES OF COCCIDIOIDAL GRANU¬ 
LOMA and ONE OF systemic blastomycosis. In Arch. Int. Med.’ v. 
16, no. 6, p. 1028-44. 
(3) Lipsitz, S. T., Lawson, G. W., and Fessenden, E. M. 
1916. a case of coccidioidal granuloma. In Jour. Amer. Med. Assoc., v. 
66, no. 18, p. 1365-1367, 4 fig. 
(4) MacNeal, W. J., and Taylor, R. M. 
1914. coccidioides immitis and coccidioidal granuloma. In Jour. Med. Re¬ 
search, v. 30, no. 3, p. 261-274, pi. 27-30. References, p. 272-273. 
