576 
Journal of Agricultural Research 
Vol. XIV, No. 13 
The results are compared in the following table: 
Antigen. 
Antigenic unit. 
Anticomple¬ 
mentary unit. 
Hemolytic 1 
action. 
Immediately after 
preparation: 
Antigen I. 
Cc. 
0. 25 
Cc. 
2. O 
None. 
Antigen II. 
' .05 
3-0 
None. 
Two weeks after 
preparation: 
Antigen I. 
•35 
None. 
Antigen II. 
. 10 
3 * 0 
None. 
The use of distilled water in laking the red blood cells had no detri¬ 
mental effect on the trypanosomes with regard to their antigenic value. 
CONCLUSIONS 
In concluding, the following advantages of the new method are pointed 
out: 
(1) The antigen is freed of all erythrocytes. 
(2) All the trypanosomes present in the blood are recovered. 
(3) The keeping quality is improved. 
(4) The time consumed is about one and one-half hours, with practi¬ 
cally no effort, as compared with four or five hours. 
(5) The antigenic power is increased and the anticomplementary 
action diminished. 
