I5« 
in the centre of the slide, was pricked with a very fine capillary tube, 
and the contents were quickly withdrawn until the egg capsule became 
transparent and collapsed. The egg contents were then emptied, 
forming a tiny drop, on tlie slide, which drop was quickly smeared into 
a thin film with a fine-pointed, cold, glass rod. The more rapidly 
these stages succeeded each other, the greater the perfection of the 
difficultly^ made film. The preparation was quickly dried at 45 0 C. 
and was then fixed in absolute alcohol before being stained by 1 
modification of Romanowsky’s method; 3 finally' the smear was 
examined with a Zeiss apochromatic 2 mm. objective and a No. 18 
compensating eyepiece, fitted to a tube length of 250mm. (x 2250) 
I he number of the spirochaetcs found varied from but three in the 
whole contents of an egg, to as high as 45 counted parasites to a field, 
with, in addition, one or more spindle-shaped collections of 
innumerable organisms. As was observed by Koch it was found that 
most spirochaetes occurred in those eggs which had been laid longest 
Morphology of Parasites occurring in the liggs (see plate X). 
As well as the typical forms of the spirochaete, occurring as th< 
did in large numbers, several peculiar varieties were also noted ai 
will be discussed in detail below. 
By far the majority of the spirochaetes were of the typical simp 
spin ar form. These varied in length from 5^ to about 20y (sing 
parasites) and were seen to occur singly and in groups of three to foi 
^ llowever> anc * this was seen in the most highly infecte 
wer _ * ,r °chaetes were arranged in remarkable collections an 
are ocnsT C !' Spiral f ° r spira1 ’ with each other. These grouf 
those sniropr Spindle ' sIla Ped by reason of the decreasing length < 
iatera,iy as "" th *■* ^ - 
U t n h boundP b“ndfe of °L h kT Se tHeSe gr ° UPed SPir0Chae “ 
nucleus blepharo j P ' Cd ^ SI11 ^ e spirochaete presents no evidence of 
lateralflager^^ no indications c 
shaped on section S & ° seems to be a spiral, possibly flat and ribbon 
deeply stained, but h termin£d P°>nt of the spirochaete is oftei 
to a point, possibly to f ° r may stain faint ly and tape 
i°ng forms of thp ° rm * terminal flagellum. In, especially. th< 
ganism, transverse, faintly-staining section: 
