452 
figs, i and /, and can be seen to be attached to the original extra- 
nuclear centrosome by faint unstaining strands, fig. At a late 
period there grows out from the new extra-nuclear centrosome a 
young flagellum, which gradually extends parallel with the old 
flagellum, as in figs. 2, 3, 6. 
At a period of the fission roughly corresponding to the above, it 
can be observed that the intra-nuclear centrosome has also divided in 
the manner represented in figs. 2, 3, and that the staining outer portion 
o the nucleus has gradually collected around, and beyond, the 
dividing portions of the intra-nuclear centrosome. In this form of 
nuclear division characteristic of Trypanosoma gambiense, no chromo¬ 
somes are formed at all, and the intra-nuclear centrosome behaves 
precise y as the so-called karyosome, or centrosome, during the 
division of the nucleus in Eug/ena, Eimeria schubergi (Schaudinn), 
and other protozoa. At a later stage the division of the intra-nudear 
centrosome becomes complete, and the outer staining portion of the 
nucleus collects round the two intra-nuclear centrosomes as in 
nurlJ’h’ 8 ’ f0rmmg t0gfether with ^^se two bodies two daughter 
nuclei having JU st the same appearance as the parent nucleus. For 
is thus n" nr 5 * 6 nUdear divisi ° n of trypanosoma gambimt 
the presence f ' * “ * ° f amitosis somewhat complicated by 
the presence of an intra-nuclear centrosome * 
the e n xt t e h n e si r a a H der the P ro «^ °f drv.sion proceeds b, 
represented in %f °,T ° new flagehtim through stages such as 
flagella are produced each encL t •” ‘ hat ‘ W °. a PP arentl >' s ‘ mila ' 
They form separate A 1 ^ a ° ex P ansi °n of the ectosarc 
of the animal's be 1 “ dU . atmg membrane s and stretch from one end 
flagella become eventually '^ 7 ^ extremities of the 
from the narrow , y parate ’ and the animal gradually splits 
daughter forn^ finally^ separatinp-^ 16 ^ ^ I5 ’ * * 
represented in figs 1 -> S ' PaSS throu g h stages such as those 
trypanosomes, each exactly reslih^ ^ ^ eventuall * v tVV ° 
During the growth and division ofl Parem f ° rm ' bU ‘ Smafc 
frequently possible tr, ^ 1 °* * le P arasit es in the blood, it is 
y Possible to find large numbers of cells in all stages of 
deSely^iatedfn 6 Cent ™e s m ? r f a ltat°”1 be n ° ted ,hat in many of the 
d to amitosis when this fo-r,/ t extra-nuclear, these bodies may be 
0 m of division occurs. 
