453 
division, and rest, wherein there exist, especially towards the narrow 
end of the cell, quantities of granules that stain under the action of 
Breinl’s stain an intense red. The colour of these granules is quite 
distinct from that of either the intra-nuclear centrosome or the extra- 
nuclear centrosome, and they cannot be stained with any satisfaction 
at all by means of the iron haematoxylin method. 1 hey are quite 
irregular in size and number, and also in their appearance, that is to 
say, they may appear in all the trypanosomes in all their stages at one 
period, and not at another. From these circumstances, we are 
inclined to regard these granules as of metabolic origin, and we can 
find no evidence that they arise from the nucleus at any time. 
These bodies should, however, not be confounded with the minute 
granules always seen, more or less throughout the spongioplasmic 
network of the entire body. These latter may, and very often do, stain 
in the same manner as the intra- and extra-nuclear centrosomes, but 
we have been unable to find any indications as to their origin, or that 
they any more than the large metabolic granules have any special 
relationship with the nucleus. 
It is possible, indeed probable, that some of these granules may 
correspond to the vegetative and trophic chromidia observed in 
Rhizopods by Schaudinn,* and by Hertwigt in Actinospheerium 
eichhorni, but owing to the very different methods we have used, we 
are not in a position to make any definite statement with respect to 
this matter. 
At a late stage of division, such as that represented in figs. 14 and 
> 3 . the appearance of the organisms at first sight very much suggests 
an act of conjugation, but in all such cases that we have examined we 
have found no indication in relation to the nuclei, or the centrosomes 
which would suggest a conjugational act. 
Along with the regular method of division just described certain 
modifications are frequently observed, which, although producing the 
most striking appearance (see figs. 8, 9, 10) are nevertheless 
apparently always capable of being explained through a disparity in 
the stage simultaneously reached by different parts of the cell. Thus 
the nucleus may divide completely and then divide again so as to form 
P- 3 * 3 - 
* Schaudinn. Arbeiten aus dem kaiserlichen Gesundheitsamte, Vol. XIX, i9°3- 
tHertwig. Archiv. fur Protistenkunde, Vol. i. ~ TT1 
aee also Mesnil. Chroniidies-et questions connexes. Bull. last., lome 11 , 
