459 
parasite of sleeping sickness have been carried, they appear thus to 
indicate a complete cycle in the blood of a single host, and the stages 
of such a life cycle can be semidiagrammatically represented in the 
manner given on page 445. 
(C) THE MORPHOLOGY AND THE MULTIPLICATION IN THE 
BLOOD OF TRYPANOSOMA BRUCEI 
The appearance of Trypanosoma brucei in the blood is 
represented in figs. 42, 43, 44, 45, 46- 1 h e chief morphological 
distinctions which the parasite of the disease Nagana presents when 
compared with Trypanosoma gambiense are found in relation to the 
distribution of the nuclear substance, and the characteis of the 
extra-nuclear centrosomes. 
The division of this trypanosome in the blood is longitudinal, as in 
the case of Trypanosoma gambiense. 1 he nucleus divides 
amitotically. The division being first marked by a lengthening of 
the extra-nuclear centrosome until this body finally separates, and to 
two minute beads. At the same time the nuclear substance also 
elongates until we observe forms such as those represented in 
%s. 43, 44, 45, 46. 
As in Trypanosoma gambiense , the stages of the division o ie 
extra-nuclear centrosome and that of the nucleus may not be the 
same, at any particular time, and through this circumstance we observe 
the same sort of multiple, and abnormal forms as in the case of 
Trypanosoma gambiense. 
(D) THE MORPHOLOGY AND THE MULTIPLICATION IN THE 
BLOOD OF TRYPANOSOMA EQUINUM 
In the blood, Trypanosoma equinum possesses much the same 
shape as that of either Trypanosoma gambiense 01 Trypanosoma 
brucei-, the nucleus is, however, usually placed neater to the b 10 ^ 
end of the cell. The extra-nuclear centrosome is large, and t e 
nuclear division which takes place during the fission of the ce s 
possesses points of much interest, since the centrosomes aie moie 
conspicuous in Trypanosoma equinum than in many other orm 
have examined. The changes which take place in the intra nuc 
centrosome during the division of the nucleus can be stu 
great clearness. 
