-— 
47i 
APPENDIX II 
MODIFICATION OF H El DEN H AIN’S HAEMATOXYLIN METHOD, 
USED DURING THE FOREGOING INVESTIGATIONS 
Fix and treat the film in the way described in Appendix I. Clear 
the slide from the alcohol containing iodine and potassium iodide, 
and pass it through successive alcohols (as in Appendix I) into water. 
Stain in a 3-t per cent, solution of iron alum for one hour, wash this 
off, and stain with the following solution : — -5 gramme haematoxylin 
dissolved in 100 c.cm. distilled water, to which, after the haematoxylin 
has dissolved, a few drops of concentrated watery solution of lithium 
carbonate is added. Stain for half an hour, and then differentiate in 
the usual way with iron alum. 
