474 
PLATE XXXIX 
Trypanosoma gambiense 
Figs, ii to 14 stained with Breinl's stain. 
Figs. 15 to 21 stained with the modification of Heidenhain’s 
haematoxylin. 
Pig. 11. Trypanosome showing division of the intra-nuclear centro- 
some, and the nuclear substance. Also multiplication of 
the intra-nuclear centrosome so as to form a group. 
Pig. 12. Trypanosome showing mode of amitotic separation of the 
nuclei. Multiplication of the intra-nuclear centrosome, 
and the formation of an independent group of these bodies 
In this and the preceding figures metabolic granules are 
also seen. 
* r 3 - Later stage in the fission of a trypanosome. The flagella 
are being detached from one another at the thin end of the 
cell. 1 he cell body is dividing from this end towards the 
other. The nuclei are already divided. Metabolic 
granules are scattered throughout the spongioplasm. 
Fif?- l 4 - Later stage in the division of a trypanosome, showing the 
manner in which the daughter cells separate. 
Fig. 15. -Trypanosome, at one of the maximum periods of the 
infection, showing a single flagellum and resting nucleus, 
and also the origin of the black line from the extra- 
nuclear centrosome. 
Fig. 16.—A similar stage wherein the black line has reached the 
neighbourhood of the nucleus. 
Fi g- 17.—The same. 
lg ' 18 ’ Tr yP anosorn e showing the black line coiled upon itse 
towards the intra-nuclear centrosome. 
'&■ ' 9 —Trypanosome showing early stages in the degeneration 0 
the black line, and its later direct association with tb 
nucleus. 
F ' S ' 20 ~ S ’ m,Iar s ‘age. wherein the intra-nuclear centrosome ha: 
become divided. 
F>g. 2J - So-called involution stage, showing resting nucleus ana 
multiplication of the intra-nuclear centrosome. 
