INTERRUPTED FEEDING 
1 o infect a susceptible animal with Trypanosoma gambiense 
the bites of Ornithodoros moubata which had fed from three to h 
minutes previously on a heavily infected animal. 
t i E £ E T: EN , T I — 1 Guinea-pig. From August 29 to October 25, 1906,362 ticks 
tea. I he blood was examined almost daily without result until January 3, m 
when the animal died of pneumonia ; no trypanosomes or signs of trypanosomas 
were seen at the autopsy. A rat sub-inoculated on November 18 did not become 
infected. 
. ^ x *’ eri ' [ ent 2. Guinea-pig. from September 27 to November 24, 1906, ;f 
6 le 00< ^ was ex amined daily until January 21, 1907 : no trypanosome 
were seen . a rat sub-inoculated on December 19 has not become infected. 
i' EN 1 IS ' oveiu B er a6> 1906, 129 ticks fed. The blood wu 
. , 6 . a most ai y up to January 21, 1907. No trypanosomes were seen. A 
at sub-inoculated on December 19 has not become infected 
These animals (Experiments 2 and 3) were inoculated in February, 
1907, with Trypanosoma gambiense. Both became infected and their 
disease ran a usual course. 
Although these experiments are far from conclusive, they seem to 
indicate that Trypanosoma gambiense is probably not easily 
transmitted by any of the common arthropods experimented with* 
The alternative explanation of the comparative insuccess of 
attempts to transmit Trypanosoma gambiense by Glossina falfaT 
as that the experiments had not been carried out under the best 
conditions. 
unless thev 9 were’f < \ Ste i tr ypanosomes could not be transmitted by tsetse flies 
He suddoVi pH tK. a tlie at some particular point in their development 
trypanosomes mnet su ^’ estlon by the observation that he could transmit cattle 
animals whose blood^ 1 \ ? tlle bites ° f tsetse flies previously fed on long-infected 
s whose blood contained very few trypanosomes. 
th SU ^ estlon course premises some sort of development of 
hap TP anos ome in the tsetse fly. From what is known of other 
_ oa there seems to be no reason why there should be none. 
Gray (i 0 , page followin S observations should be noted. Greig and 
Glossina palpalis bv the hit transmit the cattle trypanosomes conveyed by 
Nabarro and Gre.V / , (see page 20t >' 
Bruce (12 ' J 5) a so failed to transmit these trypanosomes by Stomoxyi- 
taneous inSKj& ( 2 h T rypanosoma brucei, found no case of spon- 
Hies, save Glossina, were g present St ° Ck an ‘ mals * n an area where all sorts of biting 
insects they employed**" *th«a™r Se °bservers did not try interrupted feeding. 
mechanical transmitters ■'seTbln had . W opportunity of acting » 
Gray (1 7 , succeeded in transmit “.u 810 ? , of . surra (*<>)• Minchin, Tulloch and 
ed interruptedly. ln f> the “ Jinja ” cattle trypanosome by Sfoixox) 
