IV. MORPHOLOGY OF TRYPANOSOMES IN THE 
ALIMENTARY CANAL OF CERTAIN 
ARTHROPODA* 
I. IN G LOSS!A'A /‘ALIAUS 
The observations here recorded were made on material obtained 
from dissections done in the Gambia and in the Congo in 1903 and 
the first months of 1904. Work of more immediately practical 
importance prevented us from giving as much time to this investigation 
as could have been wished, and has deferred the examination of our 
stained specimens and publication of our findings until now. The 
members of the Royal Society's Sleeping Sickness Commission and 
Koch have in the meantime published their work on the same subject 
In many points our observations coincide with theirs. For this 
reason we do not give detailed descriptions of forms observed, and 
this especially since little can be said concerning their significance. 
All our work was done with Trypanosoma gambiense and Glossina 
paipalis. 1-lies were dissected and examined in fresh and stained 
specimens (dry films) at every period from a few minutes up to eleven 
days after feeding on an infected animal. Their intestinal contents, 
muscles, ovaries, malpighian tubules, blood and salivary glands have 
een examined. Only in the alimentary canal have forms been found 
VV 1C1 can certa inly be connected with the trypanosomes. Active 
er ^ parasites were seen in the alimentary canal up 1048 hours; 
vmg, ut altered, trypanosomes up to 72 hours after feeding. No 
gnsa le trypanosomes were ever seen in the faeces of tsetse 
In ^ j ' e C * Cai excrete d per anum immediately after a fly 
has finished its feed. 
storm,H eSt !° n “ ^ a ^ over 24 hours 
whole . ' S usua % emptied of blood. In about 50 to 70 hour 
faeces S ^ 1S save f° r a small amount of greenish-t 
apnarentl ' ng:es [ i0n t ^ e trypanosomes become more active 
frequent 7 numerous i longitudinal division forms 
hXigested o aV \ bCen SCen UP t0 hours after feeding. 3 
__ Parasites^are distorted, vacuolated and granular, 
• ’ g, it must be understood that controls Here constantly examine 
