are breaking up. They seen) to be degenerating. Groups of five or 
more parasites agglomerated by their posterior extremities frequently 
occur. These usually degenerate until only shapeless debris and 
chromatic granules remain (on one occasion spherical forms were 
produced).* 
In blood ingested for some days parasites approximating to the male 
and female types 21 become relatively much more numerous than at 
first. The structure of the nucleus varies. The chromatin may be 
collected at either pole, or in three or four large masses. It may 
be irregularly arranged in transverse rods or distributed in granules 
placed on a chromatic reticulum. Sometimes the granules are 
placed about the periphery of the nucleus. Chromatophilic granules 
occur in the cytoplasm. These have been seen in positions suggesting 
that they have been extruded from the nucleus. 1 ' Occasionally one 
or perhaps two large faintly pink-staining areas, somewhat diffuse, 
but nevertheless possessing a definite outline, have occurred in the 
neighbourhood of (usually posterior to) the nucleus. This area 
suggests the chlamydoplasm 34 observed in Leucocytozoon danielewski. 
The blepharoplast often consists of two or more granules. It is 
frequently very apparent that it is placed in a “ clear area and that 
the thickened edge of the undulating membrane ends not in it but in 
a pinkish basal granule or “ diplosome." 
Trypanosomes occur which possess a third, deeply-staining 
chromatic area (one-third the size of the nucleus) in addition to 
nucleus and blepharoplast. Other polynuclear forms resembling in 
appearance the polynuclear forms described by Kochf have been seen 
(48 hours after feeding). We can say nothing concerning their 
significance, but we are not prepared to follow Koch in his interpre¬ 
tation of them. We believe that the rounded forms, which he thinks 
they produce, arise from the englobation of single trypanosomes which 
have cast off blepharoplast and undulating membrane and become 
spherical. (This process has been observed in blood within three 
hours after its ingestion.) The bodies so formed are rounded or oval, 
and measure about 40/r to you by 2'on to 3*0/1. I li e y consist of a 
hght-blue-stained protoplasm with definite contour and at first one, 
late ^ v ^ajarge and a small, chromatic masses. We have not seen 
the ,t^ ccasioi } al confluent collections of degenerated parasites are seen which recall 
Pasmodiar’ masses of Plimmer and Bradford (24)- , . „ nv 
rpiftniKi° Ch gives no measurements. We cannot say whether there is « . 
' blance »n size; our forms measure i8-o/t to 25-0/1 by you to 3-75/1. 
