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flagellum and cytoplasm move quite independently of one another. The movements 
of the cytoplasm were both amoeboid and lashing. These forms, as well as some 
very stumpy and granular, fairly round trypanosomes, may roll themselves up into 
a ball. (If the flagellum be present it is usually external.) Some seemed to be 
enclosed in some sort of a membrane, since, although motion was violent, they 
never changed their spherical shape. Others straightened out for a moment to 
immediately curl up again. In stained specimens no definite cyst wall was ever 
seen to enclose a spherical parasite. 
One apparently normal trypanosome was first seen at this time inside a large 
dear vacuolated cell of the stomach wall.* During the three and a half hours it 
was watched it remained active and unable to leave the cell. After four hours 
bacteria commenced to multiply in the specimen, and it was necessary soon after 
to discard it. No further change was observed in the spherical forms produced by 
the rounding of the above described “ pear-shaped ” parasites. 
III. IN STOMOXYS 
In the Gambia, trypanosomes, identical with those ingested, were 
found unchanged in the gut of Stomoxys up to 20 hours after they 
had fed heavily on a horse infected with Trypanosoma gambiense. 
Longitudinal divisional forms were seen. In one instance phago¬ 
cytosis (by a cell from the horse’s blood P) of a trypanosome was seen 
at this period. 
An interesting observation was made in a fly fed 18 hours previously. Two try¬ 
panosomes were attached to one another ; the separate outlines of each parasite could 
be distinguished perfectly. In about an hour and a half, the two parasites had fused 
to form a single trypanosome. The newly-formed parasite was thicker than is 
normal, had a shorter flagellum and a collection of very minute refractive granules 
at either end and no perceptible nucleus in the usual position. After twelve hours the 
parasite was motionless ; no further change had occurred. This phenomenon was 
twice seen. 
IV. IN LARVA OF AUCHMEROMYIA LUTEOLA 
The contents of the intestinal canal, the salivary glands and blood of 
larvae fed from six to thirty-four hours previously on animals infected 
with Trypanosoma gambiense were examined. The changes 
observed were very similar to those seen in the other arthropods. 
Living trypanosomes were seen in the fresh blood of the diverticulum 
up to twelve hours after feeding, and no parasites were seen in the old 
black, half-digested blood. At ten hours many parasites were dead ; 
numerous agglomerated and degenerating trypanosomes were present; 
some of the stumpier parasites were becoming rounded. The 
intestinal contents contained many bacteria ; perhaps for this reason 
comparatively few small spherical forms of the trypanosomes were 
seen. 
of/*** (quoted from 19, page 113) reports having seen intracellular for 
Dat hypanosome and of T. Itwisi (?). 
