237 
3rd, so they were then both re-inoculated intraperitoneally, one with 5 c.cm. and 
the other with 3c.cm. of a mixture of gland juice, from the same cow, and sodium 
citrate solution showing one trypanosome to five fields. 
A guinea-pig (Kxp. 142) was at the same time inoculated intraperitoneally with 
4 c.cm. of the same fluid. All three animals were carefully examined ; none ever 
became infected. 
The herd at Eala grazes on low-lying land surrounded by forest. 
Glossina pal pal is 1 exists in small numbers (July, 1904). Big game 
is scarce. 
Nouvelle Anvers, August 15th, 1904. At Nouvelle Anvers 
five of the thinnest of the 34 rattle present were examined ; two had 
trypanosomiasis. The first cattle were brought here in 1896. Trom 
then to 1900, 16 head (thirteen cows, three bulls) were imported from 
the Lower Congo (two from the Canary Islands). Since 1898, 48 
calves have been born ; ten have died (one from the results of abortion, 
nine from fever and unrecognised diseases), and several have been 
sent to other posts. Of the original animals twelve are dead (one 
slaughtered, 11 of insufficiently described diseases).* 
It is most important to note that seven of these animals came from 
Stanley Pool (Kinchassa), and all the animals sent from Kinchassa 
to Nouvelle Anvers have died in from one to four years after their 
arrival. At present there is only one animal (Bull 18, arrived Match, 
H, from Stanley Pool (Leopoldville) and it has trypanosomiasis ; 
E Broden has shown trypanosomiasis to be common among cattle 
ear Leopoldville. 
Though it cannot definitely be said that the animals from Stanley 
°°1 did not contract trypanosomiasis at Nouvelle Anvers, it is quite 
°ssible that they may have been infected with trypanosomes before 
i>eir arrival there. If this is the case the herd has contained infected 
ninials since 1899 at least. Its losses since then have certainly been 
ieav y, but the percentage of mortality is smaller and the disease seems 
ess virulent than at Eala. For instance, one cow born at Nouvelle 
Wers in 1896 and another brought there from the Canaries in the 
ai »e year are still alive and well 
J, TEE * ‘ 7 — Born here December, .902. In fair condition ; ^PP 0 ^* 0 ^ 
^lymphatic glands sligh.lv enlarged.t Fairly numerous trypanosomes 
gland juice. ' ___ 
;radu5i en ,he cause death is ill-defined, it is usually because the anima 1 ha 
- “ a Hy wasted and died. Such cases are usually ones of trypanosomiasis. 
.ry pan °" e of the animals in which a most careful examination failed o 
in,es a* 80 had slightly enlarged glands. 
