243 
puncture was done at about the sixth vertebra with a four-inch needle. About 
4C.cm. of clear fluid was obtained. Fresh preparations of the following organ 
juices and body fluids were examined, juice from the prescapular, mesenteric and 
tracheal glands, liver, spleen, marrow from long and short bones, blood, pericardial 
and cerebro-spinal fluid. Parasites were only seen in the gland preparations. 
Inoculations. — Dog* (Exp. 227), April 13, 1905. Inoculated intraperitoneally 
with about 150 c.cm. of defibrinated blood from the jugular vein. Died December 
12, 1905; no autopsy. No trypanosomes seen. 
Dog (Exp. 229), April 13, 1905. Inoculated as above with 92 c.cm. of blood. 
No trypanosomes seen until December 1, 1905, when killed because of mange. 
Dog (Exp. 229), April 13, 1905. Inoculated as above with 150c.cm. blood. 
Killed because of mange, December 1, 1905. Trypanosomes were never seen in the 
blood. 
1 ,G - 3 — Steer, Yolo, at Kasongo. Note emaciation. The type of horns 
and tendency to hump indicate a Lake Kivu origin. 
Experimental Inoculations at Kasongo 
Mosl of the animals inoculated at Kasongo were so frequently 
noculated that it has seemed best to treat the majority of them 
U -!l e, ^eparatejt eadin? __ 
Tlle d 0 g s used in all these experiments were native ones bought at Kasongo. 
