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III. OBSERVATIONS ON TRYPANOSOMES IN OTHER DOMESTIC 
ANIMALS 
Horses, Mules and Donkeys: Comparatively few of these 
animals have been brought into the part of the Congo Free State 
visited by us. Some were seen in apparently good condition at the 
following places: various localities in the Lower Congo, Leopoldville, 
Eala (three horses and a foal from Dakar present for fifteen months, 
not worked), Coquilhatville (donkeys present four to five years), 
Bamamia, four donkeys (one present for eight years), constantly worked. 
Nouvelle Anvers, a few horses here were in good condition when we 
saw them in 1904 and were still well in June, 1905. Umangi (three 
horses, a mare from Teneriffe present for five years), Lisengi (two 
horses present for six years). At Nyangwe there is a small drove of 
donkeys. None of the above animals were examined. At Kasongo 
no trypanosomes were seen in two mules and a donkey; these three 
animals had journeyed considerably in the Eastern part of the Congo 
Free State. 
In July, 1904, Commandant Sillye bought two stallions near Dakar in Senegal. 
They were then in excellent condition, and were six and twelve years old. He 
brought them to the Congo and took them on a hard journey from Kasongo almost 
to Lake Tanganyika ; from this journey they returned unloaded to Kasongo, where 
we saw them on January 14, 1905, after they had had a fortnight’s rest. Both 
animals were thin, but this was thought to be due to fatigue and bad pasturage. 
There was (Fig. 4), as in the Gambian horses (6), slight abdominal fulness without 
definite oedema. Both had rough coats from lack of grooming and were infected 
with ticks, fleas, chiggers, trombidium larvae and numerous bots. In addition both 
showed the weals of many recent fly-bites. If the animals were left to themselves 
they dropped their heads and certainly seemed to lack vigour. 
The temperature of both was about ior°F. and their glands were slightly enlarged. 
The only sign of oedema ever noticed in either horse was a swelling of the scrotum 
in one in October, 1904. The conjunctivae were pale and watery in both. 
Trypanosomes were present in good numbers in the blood of both horses. lhe 
animals were relieved of the bots, chiggers, ticks and trombidia (lotion of bichloride 
of mercury) and were given arsenic in the form of Fowler’s solution. At the en 
of January they reached Romee, where they were turned to pasture and the arsenic 
was discontinued. For two months both put on flesh. Then the older one ecame 
rapidly thinner and very weak ; the appetite remained good. It died pn 17, 
1905, after a very profuse perspiration (6). On September 14, 1905, the remaining 
borse, though comparatively thin, seemed in fair health and fed well. e scro urn 
was often swollen. 
Inoculations .—Rat (F.xp. 180). Inoculated January 14, 1905, intraperitoneally 
with a mixture of blood, from the older of the two stallions, and citrate solution 
showing 15 living trypanosomes to a cover. Parasites appeared in its 00 nex 
day and were never absent until it was accidentally killed on March 21. 
Autofsy. —Spleen much enlarged (6 - 25 x i *5 x *75 crn -)» so ^> friable , glands all 
slightly enlarged. 
