26 o 
animals carefully examined for parasites without result and believed to 
be healthy. 
The enormous difficulty of demonstrating the presence of parasites 
in animals known to be infected is well illustrated by the observations 
at Kasongo. The importance of a well-kept temperature chart is 
shown by Liboko (Chart IV), whose temperature was constantly 
elevated, although parasites were seen but once. 
Animals suspected of trypanosomiasis for any reason should be isolated: their 
temperature should be regularly and quietly taken; their gland juice and blood 
should be frequently examined, and laboratory animals should be sub-inoculated 
from them with doses of ioc.cm. of blood. An examination of this type extending 
over some months is necessary before an animal can even tentatively be said to 
be uninfected. 
VIII. SYMPTOMATOLOGY 
The symptoms are given in the order of their importance, Fever 
is practically always present. Listlessness, with progressive weakness, 
emaciation and anaemia are usually present. A tendency to diarrhoea, 
with small frequent watery stools, and enlargement of superficial 
lymphatic glands* are frequently striking features. Oedemas and 
running from the eyes and nose were seen in only one or two instances. 
Haemorrhages, eye lesions, staring of the coat and loss of hair were not 
observed. The appetite remains good to the end. In the autopsies 
done the enlargement of the lymphatic glands was by far the most 
striking feature; the glands were either pale and very watery 
(particularly superficial glands) or congested and haemorrhagic 
(particularly abdominal or mediastinal glands). Oedemas were very 
rare, exudates were scanty, the spleen was not enlarged. We saw no 
petechial haemorrhages of serous membranes. On the whole, these 
findings are identical with those of Broden. 3 
IX. COURSE OF THE DISEASE 
The disease is sometimes exceedingly chronic, and the infected 
animal may live for many months. (See Exps. 143, 146, 133. P a £ es 
2 3 ^. 239, 244, and below). 
If marked wasting continues death usually follows in both cattle 
and horses within a few weeks or three or four months. Occasional!} 
‘"i an “ na l at this stage if well cared for will recover flesh, and the 
* We are unable to say exactly 
apparent. We are inclined to 
manifestation. 
how long after infection enlarged glands beconif 
think that it may be a comparatively * ate 
