277 
in the following experiments, where sub-inoculations were made from 
experimental animals to test whether they were infected with trypano¬ 
somiasis, from i to 2 ccni. of blood were employed. 
The routine examination of the blood was made in fresh |-inch 
square coverslip preparations. Occasionally the blood of important 
animals was centrifugalised. 
For the first ten days or a fortnight after the completion of a 
treatment the animals were examined daily. As the animals lived 
longer the examinations became less and less frequent, until they 
were done approximately weekly. The atoxyl-treated controls were 
at first examined daily. When it was seen that the trypanosomes 
usually reappeared at about 16 to 25 days after the cessation of 
treatment examinations were omitted for the first two days after 
treatment. The blood of any animal evidently ill was of course 
immediately examined and, if necessary, sub-inoculations were made. 
II. STRYCHNINE 
Van Campenhout advised* that strychnine sulphate should be 
used in connection with atoxyl in the treatment of human trypano¬ 
somiasis. The following experiments were undertaken with the 
object of ascertaining whether strychnine sulphate has any action on 
trypanosomes when given alone, in conjunction with atoxyl, or when 
combined with arsenic or other drugs. Unless it is otherwise stated 
T. brucei was used in every case. 
(1) Strychnine sulphate alone 
(a) Prophylactic .— A rat was given g-ac.cm. of a i per cent, solution of strych¬ 
nine sulphate in eight doses between October 23rd and November 7t • 
inoculated with T. brucci. The infection took the usual course and the animal died 
on the seventh day after inoculation. - . . , , 
(*) Curative .— The treatment of four rats commenced on the first day of the 
appearance of parasites in the blood. Two rats got altogether 3 c.cm. o a 
oent. solution of strychnine sulphate. The remaining two rats receiv e c.c 
same solution in two doses. The rat receiving the largest dose showed sig 
°f strychnine poisoning but recovered. All the animals died on t e ir a 
showing trypanosomes. 
in the 
'In private communications, reports in the lay pr. ess Utterly^in ^ 
1 letin de 1 Academie royale de medicine de Belgique ( . strychnine is 
i n Campenhout makes it clear that he considers that , th f 
obably no t 0 n the parasite, but that it exercises a beneficial effect on the ner 
sue injured by the trypanosomes. 
