292 
Lnfortunately, our material does not permit a full description and 
explanation of the developmental phases of these nuclear structures 
We can only describe a few of the appearances observed. One 
process, however, the formation of a “ line,” seems common to all 
sexes and ages of the parasite. 1 he line is widest about the middle, 
and tapers to either extremity. It is usually gently curved; rarely 
it seems to be slightly wavy. This effect is probably produced by 
the over-lying striations of the ectoplasm. 
W e have not determined exactly how this line is produced, nor 
what is its significance. The morphological changes attending its 
appearance in the younger parasites are as follows: — The nuclear 
material, in the youngest parasites (fig. 2, 6), consists of a deeply 
chromatophilic area, connected with which is a larger pinkish area 
often containing a few chromatophilic granules. A line is projected 
apparently from the denser nuclear mass (fig. 1). At the distal end 
of this line a smaller, densely-staining area appears (fig. 3). The 
steps intervening between this stage and that shown in figs. 16, 17, 
18, where a commencing line is apparently arising from the lighter, 
not the darker, part of the chromatic material, have not been 
determined. It is possible that the forms shown in figs. 4, 5, 9. 10 
may intervene at this period. In slightly injured parasites, of all 
ages (figs. 15, 36), it is seen that the line is composed of several (up 
to four counted) filaments. Sometimes (figs. 28, 33, 33a) it may split 
longitudinally, apparently normally ; and (?) in such cases the nucleus 
sometimes also divides (fig. 39). Multiplication of the parasite has 
not been seen. This appearance is the only one observed which in 
any w ay suggests division. I he line may also divide transversely in 
the following manner. A portion of the line situated in the 
chlamydoplasmic area becomes thinned (figs. 26, 32, 40, 41).* An oval 
pink area differentiated from the rest of the nucleus surrounds this 
constriction. At either side of the constriction a dark granule 
ve ops in the line (fig. 32). Connecting these granules is a very fine 
me or axial filament.” (The “ nucleus ” in one instance (fig. 26) 
was connected to one of these granules by a line.) 
n another, slightly squashed specimen a filament connected the 
me with a differentiated “nuclear” area situated in the chlamydo- 
,hrJ h CCa ^T nally the blepharoplast 
through a defect in staining, produce 
or nucleus may lie over the line and so, 
an appearance resembling a constriction. 
