294 
the side of, above, or below the host-cell nucleus. The line is the 
most resistent part of the parasite, and is often intact when the rest 
of the parasite has been destroyed in the making of the film. 
Young forms, smaller than those illustrated (figs. 6, /, 8, 13), 
occur in which it is impossible to detect any structure beyond the 
presence of a spot of chromatin in a slightly larger area of blue 
endoplasm. Every intermediate stage occurs between these tiny 
organisms and such larger, line-bearing parasites as figs. 24, 25. 
Intermediate stages, without the line, are not seen between parasites 
of this size and the adult gametocytes. Intermediate stages with the 
line are frequent (fig. 26). The younger parasites are spherical or 
have rounded ends (figs. 4, 13, 25); as they become larger their 
extremities become pointed and affiliated. Many figures in Plate 1 
indicate that the younger parasites are amoeboid. This, and the fact 
that one or two very young parasites were seen free in the plasm, 
suggests that the parasites may be able to wander from host-cell to 
host-cell without the development of a motor apparatus. Such a 
process is described in Heemoprotcus noctuee* It is evident from 
changes in form of the adult parasites (figs. 59, 63), apparently not 
due to artefacts, that they retain up to a late stage something of the 
plasticity of the younger parasites. The younger parasites seem to 
apply themselves to (figs. 1, 4, 25) or to enter (figs. 13, 16) the host- 
cells. The larger parasites engulf them (fig. 26). 
It is noteworthy that the parasite’s endoplasm is always in close 
connection with the cell nucleus. As far as staining reaction and 
appearance goes, it is evident (figs. 6-8, 13, 21, 25) that the host-cells 
often are mononuclear white cells ; very rarely granular leucocytes 
are attached. 
I robably as the infection becomes older, the host-cell, particularly 
the nucleus, becomes larger and stains deeply (figs. 24, 26, &c). It 
is noteworthy that the nuclei of the host-cells harbouring female 
parasites are more often extruded and when present are more 
degenerated than is the case in the male parasites (figs. 62, 73 ) 
Since Romanowsky is a stain which does not penetrate, host-cell 
nuclei lying beneath the parasite are frequently almost unstained. 
In such specimens it is often difficult to make out the relative 
arrangement of host-cell and parasite. 
