3° i 
as a rule, in the “ broad ” and “ stumpy ” forms the nucleus seems 
relatively larger, of looser texture, and stains more lightly. Chromatic 
granules, number undetermined, occur in the nucleus of each type of 
trypanosome (fig. i). 
In several preparations (figs. 3, 6) two small darkly-stained and 
closely-apposed chromatophilic granules, surrounded by a pinkish 
area, occur in close connection with the nucleus, but just outside the 
nuclear membrane. In one instance a well-marked chain of oblong, 
twin chromatophilic granules runs forward, in an unstained area, from 
the anterior edge of the vacuole for about a quarter of the distance 
between the blepharoplast and the nucleus. 1 his structure recalls 
a similar appearance observed in Trypanosoma karyozcukton .* 
Whether staining deeply or lightly, the cytoplasm of the small 
trypanosomes is always granular, but the fineness of the granules 
varies greatly. Well-marked striations of the protoplasm occur in 
each form, although they are best seen in the broader forms (figs. 3 > 
4, 5). The striations are evidently a superficial structure, and are 
usually wound spirally about the parasite. In some specimens a single 
striation can be followed for almost two complete turns (figs. 4 - 5 )- 
From their arrangement it is very difficult to estimate the number of 
striations, but these seem to be about 7 to 8 (figs. 4, 5 )- 
The whole parasite is enclosed in a pink -staining periplast, which 
Qn be plainly seen at the posterior extremity and in some parasites 
all along the edge of the body, where it is unobscured by the 
undulating membrane (fig. 3). The ample undulating membrane 
seems relatively widest in the “ slender ” forms. 
The usual dimensions of each form are given in the following 
table:— 
Usual Measurements of the Small Trypanosome 
Stumpy Broad 
Form Form 
Measurement 
I ... 
1*0 ft 
i' 6 /a 
II ... 
7 '°/A 
8'o/a 
M 
Ill ... 
2'0 /A 
2-4^ ••• 
n 
IV ... 
7 ‘ 3 M 
IO' 4 /A 
V ... 
7*8 /a 
S’O^A 
breadth 
VI ... 
7 'o/a 
4'8/a 
^°tal length 
VII ... 
25T/A 
... 30-4/* 
S RENDER 
Form 
r6 /a 
IO'4/a- 
2‘0 /A 
7‘2/A 
9 - 6 /A 
2'8 /A 
30-8 /A 
* See page 2Q7 
* Dutton and Todd, 1003. Memoir XI, Liverpool School of Tropical Medicine, 
page 53 . 9 6 
T 
