307 
species of frogs and toads:— Rana galamensis (D. and B.), Rana 
oxyrhynchus (Sund.), Rana mascarensis (B.), Rappia marmorata 
Rapp.), and Bnfo regularia (Renn.). A considerable portion of them 
was infected. Blood from these infected amphibia was examined 
fresh and stained. In the fresh examinations, blood was carefully . 
taken aseptically from the heart or, if it was desired to keep the frog 
alive, either from a leg vein or a toe. Coverslip preparations were 
made and examined periodically. Blood was kept in sealed capillary 
pipettes for varying periods and was then used for making films for 
staining. Blood was taken from the frogs and, with coverslip 
preparations already made, was examined at all hours of the day and 
night. Preparations from the organs and bone marrow and of fluid 
from the body cavities were examined ; trypanosomes were seen only 
in the blood* 
Trypanosoma loricatum was present in almost every frog infected 
with trypanosomes. It was frequently associated with trypanosomes 
of any of the types described below. As a rule, it was noticed that 
parasites of the Trypanosoma sanguinis 4 type (Plate XXVI, fig. 27) 
were associated with Trypanosoma inopinalum-Wke forms and with 
the leaf-like forms 3 (Plate XXVI, fig. 28); T. mega 4 (Plate XXVII, 
figs. 35 - 39 ) occurred with forms resembling T. karyozeukton 4 
(Plate XXVIII, figs. 40-42) in everything save the absence of the 
specific chain of granules. In 99 per cent, of the frogs infected with 
trypanosomes Drepanidium was also seen to be present. As a rule, 
if there were many trypanosomes there were also many drepanidia. 
Striking exceptions to this rule were, however, seen.t 
In fresh blood T. loricatum is practically a frilled operculum of 
protoplasm, somewhat pointed at the posterior end. It is convex 
on one surface and concave on the other. From the median line of 
* In a single paper Mayer (2) described two parasites under two specific names 
loricatum (or costatum ) and rotatorium. More recent work has s t 
probably dealing with two forms of one trypanosome. One of *ese D H e d 
before disappear. We retain the name loricatum since it was o^ginally appl^ 
‘0 a parasite resembling that type of trypanosome which seems to be the adult torm 
of the haeniatozoon under discussion. A perusal of the pres P P ' 
^dent that various other forms of T. loricatum have received s^cihc names^ 
Jhese must also eventually disappear; but until the life history o P 
b «en completely worked out it is scarcely worth while discussing this poi . 
t For descriptive purposes names already given to various l 3 T es tr > 
are frequently used in this paper. They are used without question to designate 
Parasites resembling those described under these names b} 9 
