3°9 
They describe one or two anomalous forms whose position cannot be 
determined at present. Bouet 6 made cultures from the blood of frogs 
containing trypanosomes of the T. loricatum 2 and the T. rotalorium 2 
types. The results obtained with either form of parasite were 
identical Herpetomonas-like parasites (with an ill-developed undu¬ 
lating membrane, however) were the most usual forms in cultures. 
It is to be noted that every form of parasite between this type and 
the trypanosomes originally present in the frog’s blood could be seen 
in the cultures. 
Some of the parasites, directly after the preparation was made, 
were seen to have lost their striations at one or both ends and to have 
become granular. This process results in a swelling at one or both 
ends (fig. 4). Later the whole parasite becomes granular ; during this 
process a well-marked nucleus and blepharoplast are present. The 
body now becomes rounded, and the undulating membrane is gradually 
peeled off. The flagellum is attached to one end of the organism 
5 )- The parasite soon becomes completely spherical (fig. 6), and 
m this condition its diameter is about 24/i. The flagellum is still 
