3io 
attached, but soon is cast off and is seen lying in the serum, with the 
blepharoplast attached (fig. 7). Unfortunately, discarded flagella 
were never seen in stained specimens. It is, therefore, impossible to 
state whether the whole of the blepharoplast is cast off or not. If 
the whole of it is discarded a new body strongly resembling a 
blepharoplast is formed very quickly; because rounded parasites of 
all sizes from one equalling the original rounded T. loricatum to those 
only 5/z in diameter possess two chromatic areas, one the nucleus 
proper, the other resembling a blepharoplast.” 
It is suggested by some forms that part of the blepharoplast at 
least may remain when the flagellum is thrown off. Chromatophilic 
granules not infrequently occur in the cytoplasm of trypanosomes 
which probably belong to this period. Concerning their nature we 
can say nothing. 
The flagellum after being shed loses its lively movements in 
three or four seconds, and dies. The parasite is now round and 
granular with a highly refractile nucleus (fig. 8). 
In one observation the parasite lost its striations, became rounded 
and lost its flagellum in eight minutes after the preparation was made 
(figs. 9, 10).+ 
* It would be expected ( 2 ) (8) that the blepharoplast would be newly (oW* 
from the nucleus. 
t 1 he times given for the periods occupied by the various developmental c ^ 3 .■(. 
represent actual observations, and may be taken as approximate averages, 
same changes may take place more or less rapidly; very frequently the changes ti»* 
longer than has been indicated. 
