317 
day it was obviously degenerated. Some of the herpetomonas-like 
forms were seen to divide longitudinally. The further development 
of T. loricatum was not observed in fresh preparations. 
Stained specimens 
From the above-named species of frogs and toads, 220 dried films 
of blood and organ juices stained by our usual Romanowsky method 
were examined. T. loricatum vel costatum 2 was found in most of 
them (Plate XXV, figs. 1, 2, 3 and 4). The measurements of this form 
are as follows : — 
I —12 to 42 fJL. 
II.—The nucleus and blepharoplast are apparently connected. 
III. —8*2 tO l6 fl. 
IV. — 237 to 26/z. 
V.—8’2 to 16/u. 
VI. — 22 to 45/4. 
Total length of the parasite, 52 to 101^. 
It is a curious coincidence that in the twenty parasites measured, 
the length of the nucleus is approximately the same as that of the 
flagellum. 
In an organism of this size and shape, spreading and drying in the 
preparation of the film will inevitably produce a certain 
amount of distortion (Plate XXV, fig. 0 - However, the 
shape of the T. loricatum met with in Plate XXV, fig- 3 > 
corresponds with our description of the parasite seen in fresh 
blood (page 30;). The body is ovoid, the posterior extremity being 
narrower than the anterior. The organism may be found lying at full 
length, or with one end partly turned over, or completely doubled upon 
itself. The structure of the cytoplasm is finely granular. The 
periplast is pleated into ridges which run longitudinally from the 
posterior to the anterior extremity (Plate XXV, fig. 4). This appear¬ 
ance can occasionally be seen in the most beautiful manner in slightly 
disorganised slowly moving parasites, in fresh preparations. 
Occasionally the organism is twisted or folded upon itself, when the 
ndges consequently seem to run obliquely. The blepharoplast is 
usually very small and consists of four or more granules imbedded in 
a matrix. The thickened edge of the undulating membrane takes its 
origin from it and the nucleus is connected with it (Plate XX > 
u 
