329 
fhe question arises whether the cycle of multiplication just 
•• '"'il is completed in the frog, or whether it normally occurs only 
utside the frog, probably in a second blood-sucking host, as a leech. 
IV smallest rounded parasites with flagella and the herpetomonas- 
iike forms never occurred in freshly-drawn blood. Bouet 6 agrees 
with this observation in stating that none of the young parasites were 
'ceii in the blood. Franca and Athias, 6 however, record that they 
small rounded parasites both with and without flagella (probably 
terms of T. loricatum ) in blood fixed immediately after withdrawal. 
A single small rounded parasite (6// in diameter) was seen in our 
er.es of slides of freshly-drawn blood With the exception noted 
»vc, every other type of parasite occurring in the developmental 
. le described in this paper was observed in fresh-drawn blood. 
From these observations it seems that this cycle of “ swarm ” division 
may be completed in the amphibian host, but that the smallest forms 
arc rarely present in the peripheral blood. It is noteworthy in this 
amotion that there is no certain correspondence between the length 
f time blood containing 7 . loricatum has been kept and the type of 
trypanosomes present in it. 
More than once large specimens of T. loricatum were seen in 
i -i' substance red blood cells occurred. Marchoux and Salimbeni 
Mieve that the cells and the parasite are merely superimposed. 
Without wishing to assert that the cell has been ingested, that is, 
that its presence in the parasite is due to more than a mechanical 
accident, we are confident that we have seen instances where the cell 
o definitely within the cytoplasm of the trypanosome. The 
■ rvpunosomes may be attacked at any stage of their development by 
ytes Frequently they seem to resist successfully and are not 
. ted In <'iie instance a leucocyte was seen to ingest the 
haemoglobin cont txoma of a disintegrated red cell; it was 
' 'ting to note that its protoplasm contained dark brown granules 
most unusual occurrence. 
LITERATURE 
1 l.ar. e du sang. Darre, Kharkoff. 
• HU SOT les trypanosomes des Amphibiens. 
Archive, d c 1 i de Bacteriologie Camera Pestana. Tome , 
PH** » 7 and 335, U*mn« I.ibanjo da Silva. These authors give an 
C the work done on frog trypanosomes. 
