342 
TSETSE FLIES (G/ossina palpalisi 
Parasites of the type described below were found in specimens of 
Glossina palpalis freshly-caught, and at all periods, up to n days, 
after having fed on known animals infected with trypanosomes. All 
the parasites were not precisely similar, but one description will suffice 
for all. The free rod-like parasites were found only in the 
alimentary canal, nearly all of them in its anterior part. They were 
most numerous near the oesophagus, in the “ stomach or in the 
neighbouring part of the intestine. Cysts containing them were seen 
attached to, or actually in, the wall of the intestine. Some of them 
were whole, others had burst (or were burst by slight pressure), and 
from them were expelled the rod-like bodies. In fresh preparation^ 
these rods (Plate XXXII, figs, i, 2, 3, 4, 5, 8, 9, 10) were non-motile, 
they contained one or two refractile granules and they occurred singly 
or, occasionally, in small clumps. The most usual form seen in stained 
specimens was a rod about j'Op in length and VOfi in width wi 
abruptly rounded ends (figs. 1, 2, 5). It was limited by a definite 
outline, but apparently not by a capsule. Its body substance con= 
of pale blue protoplasm in which occurred chromatophilic g ranu 3 ^ 
rounded clear areas. The chromatophilic granules varied ni^ 
from extremely fine particles (fig. 6) up to masses measuring * ^ 
o'3 to 0-5 p in width (fig. 3). They were usually distributed moi ^ 
less regularly in transverse bands (figs. 1, 2, 5. 10) lying. at 
across the parasite. In such parasites the individual granule^ ^ 
barely be distinguished. Often between each band of g ranl g uC j, 
placed one of the rounded, clear areas (figs. 1, 2, 5. U ' sn]a ll 
parasites were probably preparing for transverse division. 1 ^ 
forms, apparently consisting of one or two rounded se? 1110 ’ 1 ^ j t he 
original rod-like body, were often observed (fig. 7). In xVe ll aS 
parasites showing the band-like distribution of chroniatm- d= bl ] a ter al 
in others in which pairs ol granules were arranged 111 jB jglrt 
symmetry (figs. 8, 9), it seemed as though longitudinal d lVl51 ^ s 
be about to occur. In many parasites the chromatin g r ‘‘ n } j a rg er 
distributed absolutely without order (figs. 3, 4, 6). Rarely otli^ 
and a smaller granule seemed to be in connection with eaC ue ptly 
freq 
i« e ' 
Rod-shaped parasites of all these three types wc rc ^ ($0$ 
terminated by a small chromatophilic granule (figs. 4. 5 ’ 1 ^ wh* c 
times placed in a clear area), or by a rounded enlarge 111 
