344 
perinuclear area, the irregular, non-staining almost granular areas, and 
the lines in the cytoplasm as well as the chromatophilic granules (see 
Plate XXVII, fig. 34) frequently observed in various trypanosomes 
described in this paper, are all constant phenomena, which 
seem to be of biological significance. In the development of 
T. loricatum the work of Danielewsky was confirmed and extended 
to prove a most interesting cycle of multiplication in a well-known 
trypanosome ; it must be asked whether a similar cycle may not occur 
in other trypanosomes. The rough resemblances between the 
spirochaetes seen in ulcers and in fish with previously described 
parasites of this nature are striking. In the leucocytozoon of birds an 
interesting course of development is described. * Unfortunately our 
material was too scanty to permit us to fully compare our observations 
with those of Schaudinn. Very little has been said concerning the 
drepanidia observed. In spite of a very careful search in kept pre¬ 
parations of blood, we were unable to find any indication of a direct 
relation between this parasite and the trypanosomes which were 
often associated with it. t Neither were parasites resembling 
1 rypanosoma inopinatum seen to become intracorpuscular. X In both 
stained and fresh preparations of frogs blood, however, the great 
resemblance in size and general appearance between some trypano¬ 
somes and some drepanidia was very apparent. It was also noticed 
that, as a rule, if there were many drepanidia in a given frog, there 
were also many trypanosomes. 
One of the earliest of the lessons learned from our work on these 
protozoa was the entire inadequacy of the methods of preparing 
blood-films, ordinarily employed by pathologists, for a morphological 
study of protozoa. 
The examination of dried films stained by modifications § ot 
Romanowsky’s method suffices for the purposes of clinical diagnosis. 
I his method will show many of the structures present, it was used in 
* The dangers of constructing a part of the life-history of a parasite from 
stained specimens alone are apparent. The course of development we described 
ma\ be mistaken, but the descriptions of the forms mentioned are accurate and ma) 
e relied upon so far as the defects of the method of preparation employed permit 
1 Billet, ( ulture d’un trypanosome de la grenouille chez une Hirudinee. C. R- 
Acad, des Sci., 1 . CXXXIX, Oct. .0, 1904, p 643. 
c ^ rypanosoma inopinatum et sa relation avec les Drepanidium. C. R- 
boc. de Biol., July 23, 1904, p. i6x. 
Pres! of Th erpoc) 1 ! 11 Christophers ’ The Practical Study of Malaria, page 10, University 
