THE STREPTOTHRICOSES 589 
Two days. — Veiy pale, almost Gram-negative threads, very many 
coccoid fonns and short rods, considerable segmentation of the 
longer threads. 
Three days. — Increase in short, heavy bacillary forms with bulbous 
ends, deeply stained ones and the granules being lightly Gram-positive ; 
long, slender threads are disappearing. 
Four days. — Almost exclusively short, heavy forms with bulbous 
ends with coccoid forms, heavier forms almost definitely Gram-positive, 
granules Gram-positive. 
Five days. — Much the same but more segmentation in the bacillary 
forms, coccoid forms become more numerous. 
Six days. — Individuals are somewhat longer but there are many 
rods with fusiform swellings containing granules; coccoid forms pres- 
ent in chains sometimes. 
Seven days. — More long rods or short threads, pure coccoid and 
bacillary forms. 
The morphology upon bouillon depends somewhat on age and upon 
the location. Upon the surface the long branching mycelial type appears 
early and persists until the whole surface is covered whereupon the 
segments divide into coccoid elements with metachromatic bodies. If 
heaping-up develop the coarse grains on the mass consist of granular 
or coccoid rods. When growing in the depth the coccoid form is the 
predominant one, only a few delicate myeelia, usually Gram-negative, 
being found. 
The Gram character of the organism should be emphasized. The 
young, delicate myeelia are negative or take a very feeble blue stain. 
The heavy bacillary forms are Gram-positive. Like the ray fungus 
the heavy ends are sharply Gram-positive, but unlike it, there has 
never been seen a Gram-negative bulbous capsule around this end. 
The determination of this organism was undertaken 
from the classifications of Petruschky (Kolle-Wasser- 
mann), of Castellani in Castellani and Chalmers' 
Tropical Medicine, and of the Society of American Bac- 
teriologists. In the first classification it corresponds in 
some ways with StreptotJirix liominis, and in some ways 
with Sfreptothrix caprce. As for the second authority it 
falls into the Nocardiacese, section parasitica, subsec- 
tion I, in that a distinct earthy odor is absent and that 
there is no liquefaction of coagulated protein. It 
resembles several of the species given in this subsection, 
but does not correspond exactly with any of them. Con- 
