THE STREPTOTHRICOSES 593 
involved the bone causing a pathological fracture of the lower end of 
the tibia. Smears from the periarthritis, pleuritis and blood clot in 
the bronchus show streptothrix, a short colon-like rod and a coccus in 
fours — a picture precisely like that obtained from the jaw bone cases. 
In addition to the above there is a very distinct encapsulated pneumo- 
coccus form in smears from the blood clot in the bronchus. This is 
the animal which was vaccinated Avith a culture made from the depths 
of a necrotic mass, upon which treatment she rapidly improved and 
as seen from the above notes recovered fi'om the palate condition. Why 
she should have a second infection apparently mth the same organism 
is difficult to determine. Possibly the second batch of vaccine was not 
sterile, it not having been controlled because the first batch of vaccine 
was sterile after one hour at 60° C. Possibly the animal was sensitized 
and a few bacteria settled in the leg. It was along this leg that the 
inoculations were made. 
We permit ourselves the facetious observation that 
that vaccine prevented the labial and cervical variety for 
five years, because during that period it stood in the ice- 
box, and there was no case of that particular form to 
which to give it, although a few of the nasal and gastric 
varieties occurred. It was recontrolled and did not show 
living organisms. That it should have cured the disease 
in the jaw and apparently later permitted a lighting up 
of a septicemic and pulmonary form with necroses in the 
leg is difficult to explain. 
Just recently we have used a vaccine from the current 
culture upon another case beginning in the gums and jaw 
bones. This case was detected early and was treated with 
ascending doses beginning at 0.3 mg. and running up to 
10. mg. At first there was some improvement, but the 
animal finally died from pulmonary complications. The 
course of the disease, however, instead of being three 
weeks, as is the customary duration, lasted two months, 
an extension of the course which has made us hopeful. 
These two experiments, indefinite though they be, have 
offered encouragement and seem to supply a little addi- 
tional support to the idea that the organisms stand in 
etiological relationship to the disease. 
The employment of the vaccine has been extended to 
its use as a prophylactic in animals exposed to the disease 
