408 DISEASE IN WILD MAMMALS AND BIRDS 
the operation well and the trauma had entirely disap- 
peared when the animal died on the eighth day. 
"Laboratoky Examinations. — From fluid removed 
from horse eye (No. 1) anaerobic cultures made on milk 
and blood serum, blood agar, glycerine agar ; cultures were 
made directly from the fluid, while the coagula were dried 
upon slides and stained as follows: Loeffler's, Gram's, 
Giemsa. In all there are very few recognizable bodies. 
Thej" are red blood cells, polynuclears and a very few 
small mononuclear cells. In regard to microorganisms 
three structures present themselves. A well staining 
Gram-positive, rounded end rod of fairly uniform size but 
tending to grow in pairs and stain rather irregularly with 
Loeffler and Giemsa. These forms are sometimes called 
* dumbbell ' in that they are bipolar, or even seem to have 
a constriction in their centre. Another form is peculiar 
and cannot be said to be recognized as a bacterium. It is 
circular, of fairly regular size and contour and in many 
places looks like a very large coccus. In Loeffler's stain 
it is colored deeply in the centre with a paler marginal 
zone and an unstained halo about it, which, however, is 
not hke a capsule. In the Gram and Giemsa method it is 
deeply blue or purple with a refractile centre and very 
sharply outlined contour. These forms varied from 3 
to 5 microns. The third fonn is a wavy delicate short 
mycelium-like thread. Smears from the cultures as made 
above showed chiefly a Gram-positive, rounded end rod 
but which did not grow on planting out. It grew on 
aerobic media, but was not found on anaerobic. The 
Gram-positive organism would not grow beyond the 
fourth generation. It was not identified with any known 
species by the characters manifested during the short 
time we were able to keep it alive but could be placed in 
the Hog Cholera group. The mycelium was found to be 
an aspergillus. In regard to the large coccus-like body, 
little can be added to the above description. Further 
examination did not reveal characters peimitting us to 
