THE COMMUNICABLE DISEASES 529 
relative weight of cow was too large. The finally deter- 
mined quantity was arrived at, as was the case in early 
human and bovine work, by experiment and trial and 
was as follows: A monkey of five to ten pounds (2.3 
to 4.5 kilos) received an initial dose of 1. milligram 
and for each additional five pounds (2.3 kilos) 0.5 
mg. ; this is 0.2 to 0.4 mg. per kilo. The amount given 
to man varies from 2. to 5. mg. ; if the body weigh 60 kilos 
this is 0.03 to 0.08 mg. per kilo. Cows are given usually 
in this country 400 mg. or, for a cow of 250 kilos, 1.6 mg. 
per kilo. In the early work, doses comparable to the 
figure for man failed, whereas at least two animals died 
very quickly after 1.+ mg. per kilo ; 5. mg. was the original 
high dose. While the death of a monkey after a large 
dose was of no moment and was perhaps desirable, it 
would only be the heavily diseased specimens and this 
would give no criterion upon which to judge the appro- 
priate dose for all. Experience seems to warrant us in 
continuing with our present figures since all tuberculous 
monkeys have reacted to it. Subsequent cases for retest 
are increased from 50 to 100 per cent, depending upon the 
size of the monkey, the very robust and vigorous ones 
receiving an increase represented by the higher figure. 
One monkey injected eight times has risen from 1. to 24. 
mg. with constantly a negative response over a period 
of nine years. 
Doses for Lemures are relatively higher, averaging 
1.5 mg. or about 0.5 mg. per kilo ; they are increased in 
the same manner as above. 
The Tempeeatuee Taking. 
Injections are usually made in the late forenoon, tem- 
perature records being started at the usual three PM. 
hour and continued at four-hour intervals for forty-eight 
hours, giving thirteen records over two days, a time period 
presenting two complete cycles of diurnal variation. This 
was found necessary because certain cases do not react 
