Andrews. — Annulus Ventralis. 
PLATE 45. 
Cambarus affinis. 
Fig. 15. Section of epidermis connected with connective tissue, from surface 
of a tuberosity; Flemming’s fluid followed by boiling water. 
2 X D. 
Fig. 16. Posterior face of a section posterior to figure 8 (pi. 43), showing 
posterior end of recess full of sperm and a broken passage to the 
exterior; 16 minutes after laying. 2 X A. 
Fig. 17. Vertical longitudinal section of end of recess connecting with end 
of tube over a projecting shelf. Sperms arranged as if in viscid 
streams; same series as figure 13 (pi. 44). 2 X D. 
Fig. 18. Ventral view of annulus before laying, covered with egg albumen 
and then compressed; cleared in oil of cloves. Sperms have 
spread out from suture and are held in albumen. 
Fig. 19. Surface view of sperms imbedded in glaire on the outside of a frontal 
section of the promontory of annulus fixed in Flemming’s liquid 
16 minutes after laying. Sperm that came out of median suture, 
on right edge of figure, has spread over the surface. 4 X D. 
Fig. 20. Frontal section through part of promontory, showing end of tube 
and lateral pouches of recess all full of sperm 24 hours after laying. 
2 X A. 
