BIOLOGY. 
Prot. 53 
Infection of mice with Sarcocystis muris , by feeding them with muscular 
tissue containing gymnospores, Smith (367). [It is noteworthy that the 
forms supposed to be primarily for self-infection should be capable of 
resisting the digestive juices and infecting a fresh host; as cannibalism is 
frequent among mice this may be a common mode of cross-infection, 
though whether the normal one is doubtful. In view of the importance 
of ecto-parasites in transmitting other parasites, the frequent occurrence 
of mites ( Myocoptes musculinus ) in the sub-cutis may be of more con¬ 
sequence than Smith could ascertain.] 
Dileptus ariser infected with Zoochlorellce from Stentor polymorph vs ; 
unsuccessful attempts to infect Difflugia spp. and Hyctlosphcenin sp. with 
Zoochlorellce from a different sp. of Diffluaia and from Stentor , Averintzf.y 
( 8 ). 
2. Technique. 
Doflein (88) pays considerable attention to the best methods of 
technique, for parasitic Protozoa and their culture in certain cases. 
Romanowsky’s stain (Methylene-blue-Eosin); used to distinguish uni¬ 
cellular organisms from cancerous-tissue in which they occur, Feinberg 
(109); good for encysted Arncebce, Feinberg (110); differentiates the 
nuclei of Plasmodiophora brassicce in the same manner as those of Amcebce 
etc., Feinberg (111); effect of, on ganglion cells, as contrasted with that 
on Protozoa, Feinberg (114). 
Pappenheim (281), although agreeing with Feinberg’s ideas on the 
individuality of the Protistan “ vesicular ” nucleus (obtained by relying on 
Methylene-blue and Toluidin-blue stains, see above), nevertheless believes 
that is not sufficient evidence on which to base the “ parasitic-Protozoan ” 
nature of his cancer cell-inclusions, since many liistocyte-nuclei have a 
similar elective affinity for the red constituent from those stains. 
Methods used in the study of the Tertian parasite, Argutinsky (5); 
staining technique for malarial parasites, AVright (421), Schaudinn 
(336), Giemsa (137), and Rees (310). 
A new stain for the malarial parasites, Craig (72), Reuter (313).— 
Malaria and Methylene-blue, Kunst (180).—Methylene-blue-Eosin stains 
for malarial plasmodia, R euter (312). 
Technique (staining methods) for the gametes of “ Hcemamoeha ” clani- 
lewskyi , Laveran (192). 
Technique for the Hrematozoan of piroplasmosis (. Piroplasma bigem inum ), 
Nicolle & Adil-Bey (274), Nocard & Motas (276). 
Lohmann (231) ingeniously used the branchial sac of Appendicularia 
spp., as the finest procurable net for capturing Coccolithophoridse, the 
minute forms of which would otherwise have escaped him. 
Intra-vitam staining with Loffler’s methylene-blue, for shewing up the 
plan of arrangement of the cilia in Paramcecium , Scales (333). 
3. Bibliography, etc. 
Luhe in (238) gives a useful comparative review of the recent treatises 
on Protozoa bj 7 Delage (Zool. Rec. 1896), Calkins (Zool. Rec. 1901, No. 26), 
Lang (Zool. Rec. 1901, No. 127) and Doflein (Zool. Rec. 1901, No. 62). 
Protozoan bibliography for 1901, with short abstracts, Mayer (251); 
for 1889 & 1890, with short abstracts, Prowazek (307). 
MacBride (242) criticises Rostatinski’s application of nomenclature 
laws to the Myxomycetes (Mycetozoa). 
Need of a new edition of Williamson’s monograph on the recent 
Foraminifera of Great Britain, Earland (96). 
Bibliography of t Buliminidce and t Cassidulinidce, from Italy, Fornasini 
( 121 ). 
