de Fraine.—The Seedling Structure of certain Cactaceae. 147 
and the cotyledon-strands were of equal importance in effecting the 
formation of one of the root-poles. In a third series of Echinopsis multiplex 
yet another variation was seen. The two strands derived from the larger 
cotyledon and the corresponding tubercle rotated towards one another and 
the xylems fused (c 2 , t 2f Diagram 9, Figs. 1 and 2) ; when the junction was 
almost completed the cotyledonary phloem (e 2 ) showed a belated bifurcation, 
one half immediately joining with the tubercle phloem (t 2 ) lying near it 
(Diagram 9, Fig. 3). In the smaller cotyledon (<q) the tubercle bundle (p) 
soon lost its xylem, and its phloem bifurcated. The cotyledonary trace (^) 
remained in situ, and the two phloem groups (p) passed outwards on either 
C, 
Wk 
9t> 
C, 
e, 
L \‘‘ 
Ci 
z 
Vf* 
4 = 
Diagram 9. 
Echinopsis multiplex, Series 3. 
mijm 
side of it, and fused with the cotyledonary phloem (cj, which by this time 
had bifurcated (Diagram 9, Figs 1 to 5). 
It is thus seen that a tubercle-trace may act in a manner usually 
associated with the vascular strand of a cotyledon. 
Echinopsis Lagermannii. Two vascular strands are present in each 
cotyledon. These two strands rotate towards one another, their proto- 
xylems fuse, but the phloems remain separated, one mass on either side 
of the wood ; thus a bundle is produced exactly like that figured in 
Diagram 8, Fig. 1 (<q); it is derived, however, by the fusion of two 
bundles, and not, as in Echinopsis multiplex, by the bifurcation of one. 
The behaviour of the cotyledon- and tubercle-traces in all other respects 
resembles that described in E. multiplex. Series 1. 
Echinopsis tuhiflora , Series 3, is essentially similar to E. multiplex , 
Series 1. 
In Series 2 the behaviour of the cotyledon- and tubercle-bundle of 
one seed-leaf is like that of Series 3 ; in the other seed-leaf two equal 
bundles replace the median cotyledon-bundle, and these two strands, 
together with the tubercle-bundle, act as in Echinopsis Lagermannii. 
