Germination of Helianthus annuus. 703 
sheath are yet filled with starch and it appears in the parenchyma of the 
veins for the greater part of their length in the cotyledons. 
The greater part of the proteid matter had now disappeared from the 
cells and most of them were beginning to form vacuoles. 
Stage IV. The time required for the seedlings to reach this stage 
was about ten days. Six days were required for them to reach the surface 
Fig. 4. Stage III of Seedlings. 
of the ground, after which they were placed in the C 0 2 -free apparatus and 
left four days. The cotyledons had broadened and lengthened by this 
time and the plumule had become two-leaved. See Fig. 5. The hypo- 
cotyl had a length of from 8-5 to 10 cm. from the ground to the cotyledons. 
The main roots had a length of from 6 to 7*5 cm. and had developed 
a copious growth of side roots. The starch still remained in the places 
mentioned in the previous stages, but in a much smaller quantity. The 
