Germination of Helianthus annuus. 711 
sand and the remnants that broke off in washing were carefully saved. 
Since it was found impossible to remove all the sand by washing from the 
roots of the last four stages, correction was made for the ash which was 
insoluble in nitric acid. 
Table III shows the amount of constituents present at the different 
stages examined, in grams per one hundred seeds. These results were 
obtained by multiplying the average weight of 100 parts of the seed or 
seedlings as shown in the lower part of Table III, by the average per cent, 
of the constituents on the dry weight as expressed in Table II. For example, 
the amount of ether extract in 100 cotyledons at Stage I, as shown in 
Length of Time in Days. 
Fig. 7. Relative weight of Hypocotyls and Cotyledons. In this and the following figures the 
broken lines represent the hypocotyls and the continuous lines represent the cotyledons. 
Table III, is 3-0 grams. This was obtained by multiplying 5-368, the weight 
of ico cotyledons at that period, by 55-9, the average per cent, of ether 
extract upon the dry material as shown in Table II. 
Discussion of Analytical Results. 
The interpretation of the analytical results in the preceding tables 
is aided by their diagrammatic expression in the form of curves. Diagrams 
representing the changes both in per cent, composition and in the total 
weight of constituents are given in connexion with the following discussion 
of the variation of the more important materials present in the germinating 
sunflower. 
The changes which take place in the seedling from the tenth to the 
thirteenth day, as indicated by the tables and curves, are not to be con- 
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