FLORIDA STATE HORTICULTURAL SOCIETY. 
79 
either the red or yellow Aschersonia after 
a period of five months (November 29, 
1907, to April 23, 1908). Again, spore¬ 
spraying operations in some trees of Mr. 
B. F. Hampton’s grove near Gainesville, 
made on October 6 and November 16, 
1907, resulted in promising starts of 
both the red and yellow Aschersonias. 
Spores of the red and yellow Ascher¬ 
sonias, together with some brown fun¬ 
gus material, scoured from the leaves 
with a little sand and water, were 
sprayed into some citrus trees at DeLand 
on January 17, 1908. Examination of 
the trees on April 20, 1908, revealed no 
certain growths of fungus. Introduc¬ 
tions of the red Aschersonia made into 
thirteen trees on April 21 and 22, 1908, 
at the same place, by the spore-spraying 
method have produced an excellent 
growth of this fungus [trees examined 
June 17, 1908 and paper revised to this 
date]. In two trees, Mr. H. B. Stevens 
and the writer estimated that from 30 
to 40 per cent, of the white fly larvae 
had become infected by the fungus and 
were dead. In two other trees the writer 
estimates [estimate was based on actual 
counts made upon leaves] that at least 
50 per cent, were dead. Fungus intro¬ 
duced into two other trees by the leaf- 
pinning method resulted in only very 
poor growths of fungus; the growths of 
fungus produced by the spore-spraying 
method are estimated at from several 
hundred to perhaps a thousand times as 
great as those produced by the leaf-pin¬ 
ning method in the two trees referred to. 
At the time of introducing the fungus 
the spring brood of adult “flies” had 
about disappeared and larvae of the first, 
second and third stages were in abun¬ 
dance beneath the leaves. These spray¬ 
ing and leaf-pinning operations at De- 
Land on April 21 and 2-2 are regarded as 
of great significance; because they indi¬ 
cate so clearly that the best time in 
which to introduce the red Aschersonia 
by the spore-spraying method is when 
young larvae are abundant. It appears 
that young larvae are more easily infected 
than the older ones of the fourth stage 
and pupae. The fact that rains immedi¬ 
ately followed or preceded the operations 
may also be of significance; the moisture 
favoring the germination of the spores. 
The comparatively very poor growths of 
fungus produced in the two trees into 
which leaves only had been pinned, are 
believed to indicate that the presence of 
an abundance of adult “flies” is necessary 
in order to obtain good growths of fun¬ 
gus by this method. [See: The Whitefly 
Spreads the Fungi.] What has been 
stated here for the red Aschersonia is 
also believed to hold good for the yellow 
Aschersonia; both are very similar in all 
their characters except color. The same 
principles, with one or two exceptions, 
probably apply to all the fungus parasites 
of the whitefly larvae. 
The experiments enumerated demon¬ 
strate quite clearly that the red and yel¬ 
low Aschersonias can be introduced in 
whitefly-infested trees during the fall 
months with fairly good success by the 
spore-spraying method, but leave us in 
doubt if we may ever be able to success¬ 
fully introduce them during the winter 
and early spring months. Some other 
sprayings made at Gainesville with the 
red and yellow Aschersonias and the 
brown fungus during January and 
March indicate more clearly, however, 
that this may be impossible since not a 
single growth of fungus has so far re¬ 
sulted from any of these sprayings. The 
absence of the easily infected young lar- 
