Apr. 15. 19*1 
Bacterial Spot of Tomato 
143 
more abundantly through the lower leaf epidermis, that the young leaves 
are more susceptible, and that typical infection was obtained with the 
organism from pepper. Infection of pepper and potato foliage has also 
been obtained. 
FRUIT INOCULATION 
On November 21 numerous punctures were made in a green fruit and 
two ripe fruits with a flamed needle which was frequently dipped in a 
bouillon culture of the organism. No infection occurred on the ripe 
fruits. After 11 days dark discolored areas were noted about the punc¬ 
tures in the green fruit and small blackened cores were found about such 
punctures when the surface of the fruit was sliced off. With a sterile 
scalpel, portions of this blackened tissue not in contact with the needle 
channel were removed and crushed in sterile water. I11 agar plates poured 
from this inoculum the causal organism was recovered. 
On November 20 numerous very shallow needle punctures were made 
in two green tomatoes which were suspended in moist chambers and 
sprayed with a bouillon culture. Infection occurred about most of the 
needle wounds in the shape of submerged, firm, blackened cores 2 to 3 mm. 
in diameter. 
On December 8 five green fruits and one half-ripe fruit were lightly 
punctured with a needle which was repeatedly dipped in a bouillon cul¬ 
ture. Two other green fruits were similarly wounded with a sterile 
needle. Fourteen days later infection had occurred about most of the 
punctures on four of the green fruits but not on the fifth which had 
ripened in the meantime nor on the fruit which was ripening when inocu¬ 
lated. The two controls remained free from infection. A successful 
reisolation of the organism was made from one of the inoculated fruits. 
On February 25 two green fruits were wrapped in cotton which was 
then saturated with a water suspension of the organism. By the sub¬ 
sequent addition of sterile water the cotton was kept wet four days. 
This test was later repeated, but no infection of the fruit occurred. O11 
March 25 this test was repeated with two green fruits which were punc¬ 
tured with a sterile needle before applying the cotton and inoculum. At 
the end of six days infection was evident about many of the punctures, 
and the lesions were rather shallow and more nearly resembled natural 
infection (PI. 24, F). 
On March 2 eight small and two large green fruits were punctured with 
a needle dipped in a water suspension of the organism. All showed in¬ 
fection about the needle wounds. 
In April, celluloid cylinders plugged with cotton were suspended about 
four fruit clusters to act as damp chambers. These fruits were punctured 
with a sterile needle and sprayed with a water suspension of the organism. 
At the end of 10 days infection was visible about most of the wounds. 
