i86 
Journal of Agricultural Research 
Vol. XXI, No. 3 
shiny, circular, edges entire, flat to slightly raised; buried colonies are 
0.2 to 0.5 mm. in diameter, spherical to lens-shaped, white opaque. 
Growth on agar streaks is similar, cultures several days old usually show¬ 
ing a granular structure and greenish discoloration of the medium. 
Agar stabs show only slight clouding along the puncture with abundant 
surface growth. Gelatin is liquefied, usually rapidly, various strains 
of the organsim, however, showing marked variation in rate of lique¬ 
faction. In nutrient broth there is strong turbid clouding, no surface 
growth, and more or less ropy viscid sediment in 10-day and older cul¬ 
tures. Litmus milk becomes more alkaline, and the casein is usually 
peptonized without the formation of curd. Fermentation tubes con¬ 
taining tap water, to which has been added 2 per cent peptone and 0.5 
per cent sodium chlorid plus 1 per cent, respectively, of lactose, dex¬ 
trose, saccharose, and glycerin, show no growth in the closed arm and 
no gas formation. Growth in the open arm is abundant, and litmus 
paper shows marked production of acid from glucose, less marked pro¬ 
duction of acid from saccharose, and alkali production from lactose and 
glycerin. Nitrates are not reduced. There is no growth in Cohn’s 
and Uschinsky’s solutions. Growth on cooked potato plugs is abundant 
and grayish to yellowish white with no indications of diastatic action 
on the starch. 
The group number of the organism is 211.2322033, following the third 
descriptive card of the Society of American Bacteriologists, 1 adopted in 
1907. In the classification recently proposed by the society 2 the organ¬ 
ism belongs to the genus Pseudomonas, which is also in accord with the 
system of Migula. In Smith’s 3 system it falls in the genus Bacterium. 
Pseudomonas apii, n. sp. 
A short rod, 0.44 to 0.87*1 by 0.87 to 1.74/z, one to several polar flagella; colonies on 
nutrient agar grayish white, shiny, circular, edges entire, flat to slightly raised, gran¬ 
ular with age; gelatin liquefied, litmus milk becomes more alkaline, casein peptonized 
without formation of curd; acid formed from glucose and saccharose, alkali from 
lactose and glycerin, no gas; no growth in closed arm of fermentation tubes; nitrates 
not reduced; no diastatic action; no growth in Cohn’s and Uschinsky’s solutions. 
Group number 211.232 2033. 
Parasitic in leaves of celery (Apium graveolens L.); distribution, New York, Mich¬ 
igan. 
Potebnia 4 has described a leafspot of cultivated parsley in Russia 
caused by Bacillus petroselina. The close relationship of parsley and 
1 Society of American Bacteriologists, descriptive chart. Prepared by committee on methods 
of identification of bacterial species. Endorsed by the Society for general use at the an nual meeting, Dec 
31,1907. 
1 Winslow, C., E. A., et al. the families and genera of the bacteria. Final report of the com¬ 
mittee of the Society of American bacteriologists on characterization and classification of bacterial types. 
Itl Jour. Bact., v. 5, no. 3, p. 191-229. 1920. References, p. 226-229. 
* Smith, Envin F. op. cit. 
4 Potebnia, A. A. gribnye parasity vysshikh rastenii kharkovsk. i smerzhnykh gubern. (fun¬ 
gous PARASITES OF THE HIGHER PLANTS IN KHARKOV AND ADJACENT PROVINCES. Kharkov Obi. Selskokh. 
Opyt. Sta., No. I, Eist. 1/8, p. 1-120, illus. 1915. Review by Michael Shapovalov in Phytopathology, v. 6 , 
no. 3. p. 293-295. 1916. 
