July is, 1921 Effects of Some Cucurbita Seeds on Animal Metabolism 525 
kernels. This operation also lightened the process of removing the 
hulls from the seeds. The weight of the seed consumed per day was 
taken by subtracting the weight of the hulls from the amount of seeds 
used. 
The experimental diet consisted of practically the same meals as the 
basal diet, except for the addition of pumkpin and squash seeds to the 
evening meals. On March 24, 91 gm. of pumpkin and squash seeds 
were eaten; 1 on March 25, 31 gm., and on March 26, 38 gm. The meal 
hours and study hours during this period II were exactly the same as in 
period I, and all other controllable conditions were kept as uniform as 
possible with those of period I. 
The same methods of sampling and analyzing employed in period I 
were used in period II. 
Table II. Analyses of composite samples of urine from experimental diet period 
Date. 
Volume. 
Tempera¬ 
ture. 
Specific 
gravity. 
Total 
solids. 
Acidity, a 
Ammo¬ 
nium 
hydrate 
(NHj). 
Creatinin. 
Total 
nitrogen. 
Mar. 24 b . .. . 
2 5 . 
26 . 
Cc. 
I> 195 
57 ° 
545 
°C. 
2 5 
2 5 
2 5 
I. 022 
1-037 
I. 038 
Gm. 
< 58-35 
54 - 83 
55 - 26 
415. 8 
465. I 
5 2 5 - 7 
Gm. 
I. 10274 
I. 01688 
I. 1846 
Gm. 
O.997 
. 721 
• 735 
Gm. 
9. 191 
4. 651 
7 -913 
a Expressed in cubic centimeters of N/io sodium hydroxid necessary to titrate ioo cc of sample. 
6 The slightly greater volume of urine excreted during the first day (Mar. 24) of period II as compared 
with that of March 17 of period I is ascribed to the abnormal condition resulting from the excess of pumpkin 
and squash seeds ingested at the evening meal of that day. 
The estimation of the total phosphoric acid (P 2 0 5 ) and total sulphuric 
acid (S 0 3 ) of the samples of both periods was done during the latter part 
of April. The uranium acetate method (j, p. 552) was employed for the 
determination of P 2 0 5 and Folin’s method (3, p. 346) was used for the 
estimation of S 0 3 . Table III shows the results for both periods. 
Table III .—Determination of P 2 0 5 and S 0 3 
Date of sample. 
P 2 Oc. 
S 0 3 . 
Mean of the last two 
samples. 
p 2 o 6 . 
SO3. 
Period I: 
Mar. 17. 
Gm. 
3. 229 
2-434 
2 - 535 
2. 909 
2 - 5 X 3 
2. 643 
Gm. 
I. 876 
1. 888 
i -853 
1.944 
2.321 
2. 280 
Gm. 
Gm. 
18. 
| 2. 484 
I. 870 
19. 
Period II: 
Mar. 24... 
2 < . 
} 2 - 578 
2. 300 
26. 
. 
1 About eight hours after the evening meal on March 24 acute stomach pain accompanied by eructation 
into the mouth of a very acid solution took place. To relieve this condition the back of the pharynx was 
irritated with the finger, causing vomiting. 
