Aug. is. urn Bacteriology and Chemistry of Different Silages 785 
The production of acidity appears to be due almost entirely to micro¬ 
organisms. Silage treated with 2 per cent chloroform, thus inhibiting 
growth of bacteria, showed practically no increase in acidity, while 
silage sterilized and inoculated, which process destroyed plant enzyins, 
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I ? ig. 5.—Graph showing development of amino nitrogen in Canada field pea and oat silage, 1918. 
showed an increase in acidity closely resembling that of normal silage. 
The increase in the number of acid-producing organisms usually was 
followed by an increase in acidity. 
The results of these investigations on the cause of formation of acid 
in silage agree with those of Lamb (14) and Hunter and Bushnell (jj), 
